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学科主题: 临床医学
题名:
Vaccination with immunoglobulin frame region-derived nonapeptide elicits cellular immune response against lymphoma in human leukocyte antigen-A2.1 transgenic mice
作者: Liu, Hui1; Cai, Peng2; Liu, Hong-Xing2; Wang, Jian-Ling2; Liu, Qian1; Zhu, Ping1
关键词: B-cell lymphoma ; immunoglobulin ; IgHV framework region ; cytotoxic T lymphocytes
刊名: LEUKEMIA & LYMPHOMA
发表日期: 2011-09-01
DOI: 10.3109/10428194.2011.577257
卷: 52, 期:9, 页:1795-1802
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Hematology
研究领域[WOS]: Oncology ; Hematology
关键词[WOS]: COLONY-STIMULATING FACTOR ; CYTOTOXIC T-LYMPHOCYTES ; IDIOTYPE VACCINES ; CLINICAL-TRIAL ; HEAVY-CHAIN ; PEPTIDES ; EPITOPES ; TUMOR ; PROTEINS ; CELLS
英文摘要:

The epitope in the frame region (FR) of the immunoglobulin heavy chain variable region (IgHV) is a potential target for lymphoma immunotherapy. Our previous work identified a FR-derived nonapeptide (QLVQSGAEV) capable of in vitro eliciting anti-lymphoma specific cytotoxic T lymphocytes (CTLs) in lymphocytes from human leukocyte antigen (HLA)-A2.1 donors. Here we used HLA-A2.1 transgenic mice and SCID (severe combined immunodeficiency) mice to confirm the ability of these specific CTLs against lymphomas. We immunized transgenic mice with nonapeptide conjugated to the adjuvant of PADRE (pan HLA DR-binding epitope). The specificity of the elicited CTLs from the immunized transgenic mice was identified by enzyme-linked immunospot (ELISpot) assay and pentamer staining. The elicited CTLs specifically attacked lymphomas with surface IgHV1 in vitro. Adoptive transfer of the CTLs to SCID mice loaded with QLVQSGAEV(+)/HLA-A2.1(+) lymphoma cells effectively inhibited tumor growth. Our results indicate that the relatively constant epitope in the IgHV FR may be useful for immunotherapy of lymphomas with the same IgHV subfamily.

语种: 英语
所属项目编号: 2006DFB31430 ; 2008AA02503
项目资助者: Ministry of Science &amp ; Technology of China ; National 863 High-Tech Research and Development Program of China
WOS记录号: WOS:000294228100026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56915
Appears in Collections:北京大学第一临床医学院_血液内科_期刊论文

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作者单位: 1.Dao Pei Hosp, Lab Hematol Res, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China

Recommended Citation:
Liu, Hui,Cai, Peng,Liu, Hong-Xing,et al. Vaccination with immunoglobulin frame region-derived nonapeptide elicits cellular immune response against lymphoma in human leukocyte antigen-A2.1 transgenic mice[J]. LEUKEMIA & LYMPHOMA,2011,52(9):1795-1802.
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