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学科主题: 基础医学
题名:
Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina
作者: He, Meihua1,2,3; Pan, Hong1,2,3; Xiao, Chunxia1; Pu, Mingliang1,2,3
关键词: antioxidant response element ; heme oxygenase-1 ; NADPH oxidase ; diabetic retina ; db/db mouse
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2013-06-01
DOI: 10.1167/iovs.13-12004
卷: 54, 期:6, 页:4092-4101
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: ANTIOXIDANT GENE-EXPRESSION ; PIGMENT EPITHELIAL-CELLS ; OXIDATIVE STRESS ; DB/DB MICE ; ISCHEMIA/REPERFUSION INJURY ; ENDOTHELIAL-CELLS ; HYDROGEN-PEROXIDE ; BARRIER BREAKDOWN ; PROTECTIVE ROLE ; RETINOPATHY
英文摘要:

PURPOSE. The antioxidant response element (ARE)-mediated antioxidant pathway has an important role in maintaining the redox status of the retina. The expression of ARE-mediated antioxidants, such as heme oxygenase-1 (HO-1), remains unclear in the db/db mice. We evaluated the expression of HO-1 in the retinas of db/db mice and investigated a possible role for NADPH oxidase.

METHODS. Fresh retinas were harvested from 8-, 12-, and 20-week db/db or db/m mice. Reactive oxygen species were detected by dihydroethidium. The expression levels of HO-1, Nox2, and Nox4 were evaluated by immunohistochemistry and Western blotting. In vitro retina explants culture was used to assess the role of NADPH oxidase in high glucose-induced HO-1 expression.

RESULTS. The expression of HO-1 was increased in the retinas of 8-week db/db mice, while it was decreased in 20-week db/db mice compared to age-matched controls. Similarly, the activation of Nox4 was increased in the retinas at 8 weeks and returned to basal levels at 20 weeks in db/db mice compared to age-matched controls. The activation of Nox2 was increased in the retinas of 8-, 12-, and 20-week db/db mice compared to age-matched controls. The NADPH oxidase inhibitors apocynin and DPI significantly blocked the HO-1 expression that was induced by high glucose levels in cultured retina explants.

CONCLUSIONS. The expression patterns of HO-1, Nox2, Nox4 in db/db mouse retinas, and the suppressive effects of NADPH oxidase inhibitors on the expression of HO-1 induced by high glucose levels in cultured retina explants suggest that the expression of HO-1 is, at least partially, mediated by NADPH oxidase in this diabetic animal model.

语种: 英语
所属项目编号: 2009CB320900 ; 2011CB510206 ; 30831160516 ; 81200691 ; 20090001120075
项目资助者: National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China
WOS记录号: WOS:000321120700035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56917
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Anat & Embryol, Beijing 100083, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Machine Percept, Beijing 100083, Peoples R China
3.Peking Univ, Minist Educ, Key Lab Visual Impairment & Restorat, Beijing 100083, Peoples R China

Recommended Citation:
He, Meihua,Pan, Hong,Xiao, Chunxia,et al. Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2013,54(6):4092-4101.
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