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Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina
He, Meihua1,2,3; Pan, Hong1,2,3; Xiao, Chunxia1; Pu, Mingliang1,2,3
关键词Antioxidant Response Element Heme Oxygenase-1 Nadph Oxidase Diabetic Retina Db/db Mouse
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2013-06-01
DOI10.1167/iovs.13-12004
54期:6页:4092-4101
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ANTIOXIDANT GENE-EXPRESSION ; PIGMENT EPITHELIAL-CELLS ; OXIDATIVE STRESS ; DB/DB MICE ; ISCHEMIA/REPERFUSION INJURY ; ENDOTHELIAL-CELLS ; HYDROGEN-PEROXIDE ; BARRIER BREAKDOWN ; PROTECTIVE ROLE ; RETINOPATHY
英文摘要

PURPOSE. The antioxidant response element (ARE)-mediated antioxidant pathway has an important role in maintaining the redox status of the retina. The expression of ARE-mediated antioxidants, such as heme oxygenase-1 (HO-1), remains unclear in the db/db mice. We evaluated the expression of HO-1 in the retinas of db/db mice and investigated a possible role for NADPH oxidase.

METHODS. Fresh retinas were harvested from 8-, 12-, and 20-week db/db or db/m mice. Reactive oxygen species were detected by dihydroethidium. The expression levels of HO-1, Nox2, and Nox4 were evaluated by immunohistochemistry and Western blotting. In vitro retina explants culture was used to assess the role of NADPH oxidase in high glucose-induced HO-1 expression.

RESULTS. The expression of HO-1 was increased in the retinas of 8-week db/db mice, while it was decreased in 20-week db/db mice compared to age-matched controls. Similarly, the activation of Nox4 was increased in the retinas at 8 weeks and returned to basal levels at 20 weeks in db/db mice compared to age-matched controls. The activation of Nox2 was increased in the retinas of 8-, 12-, and 20-week db/db mice compared to age-matched controls. The NADPH oxidase inhibitors apocynin and DPI significantly blocked the HO-1 expression that was induced by high glucose levels in cultured retina explants.

CONCLUSIONS. The expression patterns of HO-1, Nox2, Nox4 in db/db mouse retinas, and the suppressive effects of NADPH oxidase inhibitors on the expression of HO-1 induced by high glucose levels in cultured retina explants suggest that the expression of HO-1 is, at least partially, mediated by NADPH oxidase in this diabetic animal model.

语种英语
WOS记录号WOS:000321120700035
项目编号2009CB320900 ; 2011CB510206 ; 30831160516 ; 81200691 ; 20090001120075
资助机构National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56917
专题北京大学基础医学院
北京大学第一临床医学院_中心实验室
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Anat & Embryol, Beijing 100083, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Machine Percept, Beijing 100083, Peoples R China
3.Peking Univ, Minist Educ, Key Lab Visual Impairment & Restorat, Beijing 100083, Peoples R China
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GB/T 7714
He, Meihua,Pan, Hong,Xiao, Chunxia,et al. Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2013,54(6):4092-4101.
APA He, Meihua,Pan, Hong,Xiao, Chunxia,&Pu, Mingliang.(2013).Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,54(6),4092-4101.
MLA He, Meihua,et al."Roles for Redox Signaling by NADPH Oxidase in Hyperglycemia-Induced Heme Oxygenase-1 Expression in the Diabetic Retina".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 54.6(2013):4092-4101.
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