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学科主题: 药学
题名:
Analysis of homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn
作者: Ye, M; Guo, D; Ye, G; Huang, CG
刊名: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
发表日期: 2005-02-01
DOI: 10.1016/j.jasms.2004.11.007
卷: 16, 期:2, 页:234-243
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Chemistry, Physical ; Spectroscopy
研究领域[WOS]: Chemistry ; Spectroscopy
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRIC DETECTION ; ELECTROSPRAY-IONIZATION ; SUBTERRANEAN PART ; KER-GAWLER ; CONSTITUENTS ; FLAVONOIDS ; TUBER ; IDENTIFICATION ; FRAGMENTATION
英文摘要:

The homoisoflavonoids in Ophiopogon japonicus (Thunb.) Ker, Gawler were analyzed by high-performance liquid chromatography-diode array detection-electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MSn). Homoisoflavonoids gave prominent [M - H] ions by electrospray ionization monitored in the negative ion mode. They could be classified into two types depending on the fragmentation behavior of their [M - H](-) ions in the ion trap mass analyzer. The [M - H](-) ions of homoisoflavonoids with a saturated C2-3, bond underwent C3-9 bond cleavage to lose the B-ring, which was followed by the loss of a molecule of CO. The [M - H](-) ions of homoisoflavonoids with a C2-3 double bond usually eliminated a CO molecule first, and then underwent the cleavage Of C3-9 or C9-1 bonds. For homoisoflavonoids with a C-6 formyl group, however, the neutral loss of CO was the first fragmentation step; the presence of a methoxyl group at C-8 could lead to the cleavage of C-ring. No retro Diels-Alder (RDA) fragmentation characteristic for normal flavonoids was observed. The above fragmentation rules were reported for the first time, and were implemented for the analysis of homoisoflavonoids in O. japonicus. The CHCl3-MeOH extract was separated on a Zorbax Extend-C-18 column, eluting with a acetonitrile-0.3% acetic acid gradient. A total of 18 homoisoflavonoids, including seven new minor constituents, were identified or tentatively characterized based on the UV spectra and tandem mass spectra of the HPLC peaks. (C) 2004 American Society for Mass Spectrometry.

语种: 英语
WOS记录号: WOS:000226999700011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56925
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China

Recommended Citation:
Ye, M,Guo, D,Ye, G,et al. Analysis of homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2005,16(2):234-243.
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