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学科主题: 基础医学
题名:
miR-200b targets GATA-4 during cell growth and differentiation
作者: Yao, Chun-Xia1; Wei, Qing-Xia1; Zhang, Yan-Yan2; Wang, Wei-Ping3; Xue, Li-Xiang3; Yang, Fen4; Zhang, Shu-Feng1; Xiong, Cheng-Juan1; Li, Wen-Yan1; Wei, Zhi-Ru1; Zou, Yunzeng5,6; Zang, Ming-Xi1
关键词: GATA-4 ; miR-200b ; transcription regulation ; cell growth ; cell differentiation
刊名: RNA BIOLOGY
发表日期: 2013-04-01
DOI: 10.4161/rna.24370
卷: 10, 期:4, 页:465-480
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CARDIAC-MUSCLE-CELLS ; GENE-EXPRESSION ; STEM-CELLS ; PROLIFERATION ; MYOCYTES ; NKX2.5
英文摘要:

GATA-4 is an important transcription factor involved in several developmental processes of the heart, such as cardiac myocyte proliferation, differentiation and survival. The precise mechanisms underlying the regulation of GATA-4 remain unclear, this is especially true for the mechanisms that mediate the post-transcriptional regulation of GATA-4. Here, we demonstrate that miR-200b, a member of the miR-200 family, is a critical regulator of GATA-4. Overexpression of miR-200b leads to the downregulation of GATA-4 mRNA and a decrease in GATA-4 protein levels. Moreover, miR-200b not only inhibits cell growth and differentiation but also reverses the growth response mediated by GATA-4, whereas depletion of miR-200b leads to a slight reversal of the anti-growth response achieved by knocking down endogenous GATA-4. More importantly, the cell cycle-associated gene cyclin D1, which is a downstream target of GATA-4, is also regulated by miR-200b. Thus, miR-200b targets GATA-4 to downregulate the expression of cyclin D1 and myosin heavy chain (MHC), thereby regulating cell growth and differentiation.

语种: 英语
所属项目编号: 2007AA02Z122 ; 2012AA022501 ; 81141044 ; 31071206 ; 082102310024 ; 104300510016
项目资助者: Ministry of Science and Technology of China (863 Program) ; National Natural Science Foundation of China ; International Cooperation and Exchange Foundation of Henan Scientific Committee ; Scientific Research Foundation for the Returned Overseas Chinese Scholars ; State Education Ministry (SRF for ROCS, SEM) ; Ministry of Personnel ; Henan University Key Teacher Foundation
WOS记录号: WOS:000321701600002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56926
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Zhengzhou Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Zhengzhou 450052, Peoples R China
2.Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450052, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
4.China Astronaut Res & Training Ctr, Dept Biochem & Mol Biol, State Key Lab Space Med Fundamentals & Applicat, Beijing, Peoples R China
5.Fudan Univ, Zhongshan Hosp, Shanghai Inst Cardiovasc Dis, Shanghai 200433, Peoples R China
6.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

Recommended Citation:
Yao, Chun-Xia,Wei, Qing-Xia,Zhang, Yan-Yan,et al. miR-200b targets GATA-4 during cell growth and differentiation[J]. RNA BIOLOGY,2013,10(4):465-480.
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