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学科主题: 临床医学
题名:
microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries
作者: Zhang, Huan1; Jiang, Xiaohua1; Zhang, Yuanwei1; Xu, Bo1; Hua, Juan1; Ma, Tieliang1; Zheng, Wei1; Sun, Rui1; Shen, Wei2; Cooke, Howard J.1,3; Hao, Qiaomei1; Qiao, Jie4; Shi, Qinghua1
刊名: REPRODUCTION
发表日期: 2014-07-01
DOI: 10.1530/REP-13-0508
卷: 148, 期:1, 页:43-54
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: CELL NUCLEAR ANTIGEN ; FORM PRIMORDIAL FOLLICLES ; GERM-CELL ; MESSENGER-RNA ; IN-VITRO ; TRANSLATIONAL REPRESSION ; DNA-REPLICATION ; GRANULOSA-CELLS ; APOPTOSIS ; EXPRESSION
英文摘要:

In mammals, the primordial follicle pool, providing all oocytes available to a female throughout her reproductive life, is established perinatally. Dysregulation of primordial follicle assembly results in female reproductive diseases, such as premature ovarian insufficiency and infertility. Female mice lacking Dicer1 (Dicer), a gene required for biogenesis of microRNAs, show abnormal morphology of follicles and infertility. However, the contribution of individual microRNAs to primordial follicle assembly remains largely unknown. Here, we report that microRNA 376a (miR-376a) regulates primordial follicle assembly by modulating the expression of proliferating cell nuclear antigen (Pcna), a gene we previously reported to regulate primordial follicle assembly by regulating oocyte apoptosis in mouse ovaries. miR-376a was shown to be negatively correlated with Pcna mRNA expression in fetal and neonatal mouse ovaries and to directly bind to Pcna mRNA 3′ untranslated region. Cultured 18.5 days postcoitum mouse ovaries transfected with miR-376a exhibited decreased Pcna expression both in protein and mRNA levels. Moreover, miR-376a overexpression significantly increased primordial follicles and reduced apoptosis of oocytes, which was very similar to those in ovaries co-transfected with miR-376a and siRNAs targeting Pcna. Taken together, our results demonstrate that miR-376a regulates primordial follicle assembly by modulating the expression of Pcna. To our knowledge, this is the first microRNA-target mRNA pair that has been reported to regulate mammalian primordial follicle assembly and further our understanding of the regulation of primordial follicle assembly.

语种: 英语
所属项目编号: 2013CB947900 ; 2012CB944402 ; 2011CB944501 ; 2013CB945502 ; KSCX2-EW-R-07
项目资助者: National Basic Research Program of China (973) ; Knowledge Innovation Program of the Chinese Academy of Sciences
WOS记录号: WOS:000338536800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56933
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
2.Qingdao Agr Univ, Lab Germ Cell Biol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao, Shandong, Peoples R China
3.Univ Edinburgh, Human Genet Unit, MRC, Inst Genet & Mol Med, Edinburgh EH8 9YL, Midlothian, Scotland
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Huan,Jiang, Xiaohua,Zhang, Yuanwei,et al. microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries[J]. REPRODUCTION,2014,148(1):43-54.
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