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学科主题: 临床医学
题名:
Label-free fluorescence turn on detection of tiopronin with tunable dynamic range based on the ensemble of Alizarin Red S/copper ion
作者: Chen, Zhanguang1; Wang, Zhen1; Chen, Junhui2; Gao, Wenhua1,3
关键词: Tiopronin ; Turn on ; Label-free ; Alizarin Red S ; Copper ions
刊名: TALANTA
发表日期: 2012-09-15
DOI: 10.1016/j.talanta.2012.07.021
卷: 99, 页:774-779
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; PIEZOELECTRIC IMMUNOSENSORS ; HUMAN PLASMA ; THIOL-GROUP ; ELECTROCHEMICAL BIOSENSORS ; DERIVATIZATION REAGENT ; METHYL ACRYLATE ; ASSAY ; CISPLATIN ; ACID
英文摘要:

In this study, a new type of rapid, label-free fluorescence turn-on assay for detection of tiopronin using Alizarin Red S (ARS)/copper ion ensemble is developed. ARS is high fluorescence in BR buffer solution. But, the fluorescence of ARS can be significantly quenched by copper ions due to ground-state complexation. However, in the presence of tiopronin, copper ions were released from the ARS and thus restored the fluorescence of ARS. The assay has several important features. First, the system is simple in design, fast in operation and is more convenient and promising than other methods. Second, the proposed assay eliminated the separation process and sophisticated instrumentations. Third, the detection process can be seen with the naked eye and can be easily adapted to automated high-throughput screening. At last, the assay has high sensitivity and selectivity for tiopronin and the detection limit is 0.8 ng/mL which is lower than or at least comparable to the previous methods. Moreover, the dynamic range of the sensor can be tuned simply by adjusting the concentration of copper ions. Importantly, the protocol offers high selectivity for the determination of tiopronin in pharmaceutical tablets, injection and biological samples with satisfactory results. Thus, the assay shows great potential applications in the fields of pharmaceuticals and clinical analysis. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 9151022501000019 ; YR09009
项目资助者: Natural Science Foundation of Guangdong Province ; Science Foundation of Shantou University
WOS记录号: WOS:000309785300110
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56953
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China
3.Shantou Univ, Anal & Testing Ctr, Shantou 515063, Guangdong, Peoples R China

Recommended Citation:
Chen, Zhanguang,Wang, Zhen,Chen, Junhui,et al. Label-free fluorescence turn on detection of tiopronin with tunable dynamic range based on the ensemble of Alizarin Red S/copper ion[J]. TALANTA,2012,99:774-779.
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