IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies
Fang, M; Jiang, X; Yang, Z; Yin, CC; Li, H; Zhao, R; Zhang, Z; Lin, Q; Huang, HL
关键词Single-chain Antibody Bispecific Antibody Single-chain Bispecific Antibody Interlinker
刊名CHINESE SCIENCE BULLETIN
2003-11-01
DOI10.1360/03wc0168
48期:21页:2277-2283
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]THERAPY ; DIABODY ; TETRAVALENT ; RETENTION ; MOLECULES ; BIVALENT ; HER2/NEU ; CANCER ; FV
英文摘要

Single-chain bispecific antibody (scBsAb) is one of the promising genetic engineering antibody formats for clinical application. But the effects of interlinker sequences on the biological properties of bispecific single-chain antibodies have not been studied in deta it. Three interlinker sequences were designed and synthesized, and denominated as Fc, HSA, 205C′, respectively. Universal vectors with these different interlinker sequences for scBsAb expression in E. coli were constructed. A model scBsAb based on a reshaped single-chain antibody (scFv) against human CD3 and a scFv directed against human ovarian carcinoma were generated and expressed in E. coli. The results of SDS-PAGE and Western blot showed that the different interlinker sequences did not affect the expression level of scBsAb. However, as demonstrated by ELISA and pharmacokinetics studies performed in juice, scBsAbs with different interlinker sequences had difference in the antigen-binding activities and terminal half-life time (T-1/2beta) in vivo, the interlinker HSA could remarkably prolong the retention time of scBsAb in blood. These results indicated that the peptide sequence of interlinker could affect important biological properties of scBsAb, such as antigen-binding properties and stability ill vivo. So, selection of an appropriate interlinker sequence is very important for scBsAb construction. Optimal interlinker can bring scBsAb biological properties more suitable for clinical application.

语种英语
WOS记录号WOS:000186533700002
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56958
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Beijing Canc Hosp, Beijing 100036, Peoples R China
3.Acad Sinica, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Fang, M,Jiang, X,Yang, Z,et al. Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies[J]. CHINESE SCIENCE BULLETIN,2003,48(21):2277-2283.
APA Fang, M.,Jiang, X.,Yang, Z.,Yin, CC.,Li, H.,...&Huang, HL.(2003).Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies.CHINESE SCIENCE BULLETIN,48(21),2277-2283.
MLA Fang, M,et al."Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies".CHINESE SCIENCE BULLETIN 48.21(2003):2277-2283.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fang, M]的文章
[Jiang, X]的文章
[Yang, Z]的文章
百度学术
百度学术中相似的文章
[Fang, M]的文章
[Jiang, X]的文章
[Yang, Z]的文章
必应学术
必应学术中相似的文章
[Fang, M]的文章
[Jiang, X]的文章
[Yang, Z]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。