IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies
Fang, M; Jiang, X; Yang, Z; Yin, CC; Li, H; Zhao, R; Zhang, Z; Lin, Q; Huang, HL
关键词Single-chain Antibody Bispecific Antibody Single-chain Bispecific Antibody Interlinker
刊名CHINESE SCIENCE BULLETIN
2003-11-01
DOI10.1360/03wc0168
48期:21页:2277-2283
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]THERAPY ; DIABODY ; TETRAVALENT ; RETENTION ; MOLECULES ; BIVALENT ; HER2/NEU ; CANCER ; FV
英文摘要

Single-chain bispecific antibody (scBsAb) is one of the promising genetic engineering antibody formats for clinical application. But the effects of interlinker sequences on the biological properties of bispecific single-chain antibodies have not been studied in deta it. Three interlinker sequences were designed and synthesized, and denominated as Fc, HSA, 205C′, respectively. Universal vectors with these different interlinker sequences for scBsAb expression in E. coli were constructed. A model scBsAb based on a reshaped single-chain antibody (scFv) against human CD3 and a scFv directed against human ovarian carcinoma were generated and expressed in E. coli. The results of SDS-PAGE and Western blot showed that the different interlinker sequences did not affect the expression level of scBsAb. However, as demonstrated by ELISA and pharmacokinetics studies performed in juice, scBsAbs with different interlinker sequences had difference in the antigen-binding activities and terminal half-life time (T-1/2beta) in vivo, the interlinker HSA could remarkably prolong the retention time of scBsAb in blood. These results indicated that the peptide sequence of interlinker could affect important biological properties of scBsAb, such as antigen-binding properties and stability ill vivo. So, selection of an appropriate interlinker sequence is very important for scBsAb construction. Optimal interlinker can bring scBsAb biological properties more suitable for clinical application.

语种英语
WOS记录号WOS:000186533700002
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56958
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Beijing Canc Hosp, Beijing 100036, Peoples R China
3.Acad Sinica, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Fang, M,Jiang, X,Yang, Z,et al. Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies[J]. CHINESE SCIENCE BULLETIN,2003,48(21):2277-2283.
APA Fang, M.,Jiang, X.,Yang, Z.,Yin, CC.,Li, H.,...&Huang, HL.(2003).Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies.CHINESE SCIENCE BULLETIN,48(21),2277-2283.
MLA Fang, M,et al."Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies".CHINESE SCIENCE BULLETIN 48.21(2003):2277-2283.
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