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学科主题基础医学
Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs
Wang, Xianwei1; Chen, Xi1; Tang, Zhiyu1; Yao, Weijuan1; Liu, Xiao1; Wei, Risheng1; Wang, Xifu2; Ka, Weibo3; Sun, Dagong3; He, Dongqi1; Wen, Zongyao1; Chien, Shu4
关键词Ryanodine receptor Red blood cell Calcium ions RBC deformability Membrane microviscosity
刊名JOURNAL OF BIOMECHANICS
2009-12-11
DOI10.1016/j.jbiomech.2009.07.036
42期:16页:2774-2779
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biophysics ; Engineering, Biomedical
资助者National Natural Science Foundations of China ; National Natural Science Foundations of China
研究领域[WOS]Biophysics ; Engineering
关键词[WOS]CALCIUM-RELEASE CHANNELS ; SARCOPLASMIC-RETICULUM ; SKELETAL-MUSCLE ; CELLULAR DEFORMABILITY ; ERYTHROCYTES ; PERMEABILIZATION ; INACTIVATION ; ACTIVATION ; DEPENDENCE ; CELLS
英文摘要

Ryanodine receptors (RyRs) are a family of Ca2+ channel proteins that mediate the massive release of Ca2+ from the endoplasmic reticulum into the cytoplasma. In the present study, we manipulated the incorporation of RyR1 into RBC membrane and investigated its influences on the intracellular Ca2+ ([Ca2+](in)) level and the biomechanical properties in RBCs. The incorporation of RyR1 into RBC membranes was demonstrated by both immunofluorescent staining and the change of [Ca2+](in) of RBCs. In the presence of RyR1, [Ca2+](in) showed biphasic changes, i.e., it increased with the extracellular Ca2+ ([Ca2+](ex)) up to 5 mu M and then decreased with the further increase of [Ca2+](ex). However, [Ca2+](in) remained constant in the absence of the RyR1. The results of biomechanical measurements on RBCs, including deformability, osmotic fragility, and membrane microviscosity, reflected similar biphasic changes of [Ca2+](in) mediated by RyR1 with the increases of [Ca2+](ex). Therefore, it is believed that RyR1 can incorporate into RBC membrane in vitro, and mediate Ca2+ influx, and then regulate RBC biomechanical properties. This information suggests that RBCs may serve as a model to study the function of RyR1 as a Ca2+ release channel. (C) 2009 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号10572007 ; 30770532
资助者National Natural Science Foundations of China ; National Natural Science Foundations of China
WOS记录号WOS:000273135200023
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56964
专题基础医学院_生物物理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
2.Peking Univ, Shougang Hosp, Dept Cardiol, Beijing 100144, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Beijing 100191, Peoples R China
4.Univ Calif San Diego, Dept Bioengn & Med, La Jolla, CA 92093 USA
推荐引用方式
GB/T 7714
Wang, Xianwei,Chen, Xi,Tang, Zhiyu,et al. Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs[J]. JOURNAL OF BIOMECHANICS,2009,42(16):2774-2779.
APA Wang, Xianwei.,Chen, Xi.,Tang, Zhiyu.,Yao, Weijuan.,Liu, Xiao.,...&Chien, Shu.(2009).Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs.JOURNAL OF BIOMECHANICS,42(16),2774-2779.
MLA Wang, Xianwei,et al."Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs".JOURNAL OF BIOMECHANICS 42.16(2009):2774-2779.
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