北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生物物理学系  > 期刊论文
学科主题: 基础医学
题名:
Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs
作者: Wang, Xianwei1; Chen, Xi1; Tang, Zhiyu1; Yao, Weijuan1; Liu, Xiao1; Wei, Risheng1; Wang, Xifu2; Ka, Weibo3; Sun, Dagong3; He, Dongqi1; Wen, Zongyao1; Chien, Shu4
关键词: Ryanodine receptor ; Red blood cell ; Calcium ions ; RBC deformability ; Membrane microviscosity
刊名: JOURNAL OF BIOMECHANICS
发表日期: 2009-12-11
DOI: 10.1016/j.jbiomech.2009.07.036
卷: 42, 期:16, 页:2774-2779
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Engineering, Biomedical
研究领域[WOS]: Biophysics ; Engineering
关键词[WOS]: CALCIUM-RELEASE CHANNELS ; SARCOPLASMIC-RETICULUM ; SKELETAL-MUSCLE ; CELLULAR DEFORMABILITY ; ERYTHROCYTES ; PERMEABILIZATION ; INACTIVATION ; ACTIVATION ; DEPENDENCE ; CELLS
英文摘要:

Ryanodine receptors (RyRs) are a family of Ca2+ channel proteins that mediate the massive release of Ca2+ from the endoplasmic reticulum into the cytoplasma. In the present study, we manipulated the incorporation of RyR1 into RBC membrane and investigated its influences on the intracellular Ca2+ ([Ca2+](in)) level and the biomechanical properties in RBCs. The incorporation of RyR1 into RBC membranes was demonstrated by both immunofluorescent staining and the change of [Ca2+](in) of RBCs. In the presence of RyR1, [Ca2+](in) showed biphasic changes, i.e., it increased with the extracellular Ca2+ ([Ca2+](ex)) up to 5 mu M and then decreased with the further increase of [Ca2+](ex). However, [Ca2+](in) remained constant in the absence of the RyR1. The results of biomechanical measurements on RBCs, including deformability, osmotic fragility, and membrane microviscosity, reflected similar biphasic changes of [Ca2+](in) mediated by RyR1 with the increases of [Ca2+](ex). Therefore, it is believed that RyR1 can incorporate into RBC membrane in vitro, and mediate Ca2+ influx, and then regulate RBC biomechanical properties. This information suggests that RBCs may serve as a model to study the function of RyR1 as a Ca2+ release channel. (C) 2009 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 10572007 ; 30770532
项目资助者: National Natural Science Foundations of China
WOS记录号: WOS:000273135200023
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56964
Appears in Collections:基础医学院_生物物理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical.pdf(460KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
2.Peking Univ, Shougang Hosp, Dept Cardiol, Beijing 100144, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Beijing 100191, Peoples R China
4.Univ Calif San Diego, Dept Bioengn & Med, La Jolla, CA 92093 USA

Recommended Citation:
Wang, Xianwei,Chen, Xi,Tang, Zhiyu,et al. Ryanodine receptor 1 mediates Ca2+ transport and influences the biomechanical properties in RBCs[J]. JOURNAL OF BIOMECHANICS,2009,42(16):2774-2779.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Xianwei]'s Articles
[Chen, Xi]'s Articles
[Tang, Zhiyu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Xianwei]‘s Articles
[Chen, Xi]‘s Articles
[Tang, Zhiyu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace