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学科主题: 药学
题名:
A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method
作者: Zhang, Peng; Yang, Xiu-Wei1
关键词: nodakenetin ; 3 &prime ; (R)-hydroxy-nodakenetin-3 &prime ; -ol ; 3 &prime ; (S)-hydroxy-nodakenetin-3 &prime ; -ol ; rat microsome ; RP-HPLC
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2010-02-01
DOI: 10.1002/bmc.1276
卷: 24, 期:2, 页:216-221
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: COUMARIN-GLYCOSIDES ; CHROMATOGRAPHY ; DRUG
英文摘要:

The biotransformation of nodakenetin (NANI) by rat liver microsomes in vitro was investigated. Two major polar metabolites were produced by liver microsomes from phenobarbital-pretreated rats and detected by reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. The chemical structures of two metabolites were firmly identified as 3′(R)-hydroxy-nodakenetin-3′-ol and 3′(S)-hydroxy-nodakenetin-3′-ol, respectively, on the basis of their H-1-NMR, MS and optical rotation analysis. The latter was a new compound. A sensitive, selective and simple RP-HPLC method has been developed for the simultaneous determination of NANI and its two major metabolites in rat liver microsomes. Chromatographic conditions comprise a C-18 column, a mobile phase with MeOH-H2O (40:60, v/v), a total run time of 40 min, and ultraviolet absorbance detection at 330 nm. In the rat heat-inactivated liver microsomal supernatant, the lower limits of detection and quantification of metabolite I, metabolite II and NANI were 5.0, 2.0, 10.0 ng/mL and 20.0, 5.0, 50.0 ng/mL, respectively, and their calibration curves were linear over the concentration range 50-400, 20-120 and 150-24000 ng/mL, respectively. The results provided a firm basis for further evaluating the pharmacokinetics and clinical efficacy of NANI. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2006BA106A01-02
项目资助者: National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program of China
WOS记录号: WOS:000273972600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56977
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Peng,Yang, Xiu-Wei. A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method[J]. BIOMEDICAL CHROMATOGRAPHY,2010,24(2):216-221.
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