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A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method
Zhang, Peng; Yang, Xiu-Wei1
关键词Nodakenetin 3 &Prime (r)-hydroxy-nodakenetin-3 &Prime -ol 3 &Prime (s)-hydroxy-nodakenetin-3 &Prime -ol Rat Microsome Rp-hplc
刊名BIOMEDICAL CHROMATOGRAPHY
2010-02-01
DOI10.1002/bmc.1276
24期:2页:216-221
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]COUMARIN-GLYCOSIDES ; CHROMATOGRAPHY ; DRUG
英文摘要

The biotransformation of nodakenetin (NANI) by rat liver microsomes in vitro was investigated. Two major polar metabolites were produced by liver microsomes from phenobarbital-pretreated rats and detected by reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. The chemical structures of two metabolites were firmly identified as 3′(R)-hydroxy-nodakenetin-3′-ol and 3′(S)-hydroxy-nodakenetin-3′-ol, respectively, on the basis of their H-1-NMR, MS and optical rotation analysis. The latter was a new compound. A sensitive, selective and simple RP-HPLC method has been developed for the simultaneous determination of NANI and its two major metabolites in rat liver microsomes. Chromatographic conditions comprise a C-18 column, a mobile phase with MeOH-H2O (40:60, v/v), a total run time of 40 min, and ultraviolet absorbance detection at 330 nm. In the rat heat-inactivated liver microsomal supernatant, the lower limits of detection and quantification of metabolite I, metabolite II and NANI were 5.0, 2.0, 10.0 ng/mL and 20.0, 5.0, 50.0 ng/mL, respectively, and their calibration curves were linear over the concentration range 50-400, 20-120 and 150-24000 ng/mL, respectively. The results provided a firm basis for further evaluating the pharmacokinetics and clinical efficacy of NANI. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000273972600014
项目编号30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; Z0004105040311 ; 2006BA106A01-02
资助机构National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Beijing Municipal Special-Purpose Science Foundation of China ; National Sciences and Technology Program of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56977
专题北京大学药学院_天然药物学系
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Peng,Yang, Xiu-Wei. A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method[J]. BIOMEDICAL CHROMATOGRAPHY,2010,24(2):216-221.
APA Zhang, Peng,&Yang, Xiu-Wei.(2010).A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method.BIOMEDICAL CHROMATOGRAPHY,24(2),216-221.
MLA Zhang, Peng,et al."A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method".BIOMEDICAL CHROMATOGRAPHY 24.2(2010):216-221.
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