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Mechanism for transfer of transposon Tn2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution
Zhou, Wenqing1; Yao, Kaihu2,3; Zhang, Gang1; Yang, Yonghong2,3; Li, Yun4; Lv, Yuan4; Feng, Jie1,5
关键词Transformation Recombination Fitness Cost
刊名JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
2014-06-01
DOI10.1093/jac/dku019
69期:6页:1470-1473
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences
研究领域[WOS]Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
关键词[WOS]ELEMENTS ; ERM(B) ; MEF(E) ; ASSOCIATION
英文摘要

The objective of this study was to identify the mechanism responsible for the horizontal transfer of transposon Tn2010 in Streptococcus pneumoniae, and the genomic alterations introduced by the transfer process.

Tn2010 was identified using PCR in 15 clinical isolates of S. pneumoniae with erythromycin resistance. S. pneumoniae and Enterococcus faecalis isolates were used as recipient cells in mating and transformation experiments to test the conjugative transferability and transformability of Tn2010. Whole-genome sequencing was used to assess the effects of the Tn2010 transfer on recipient genomes. The biological cost of the horizontal acquisition of Tn2010 and additional genomic changes was investigated by growth competition experiments.

Tn2010 was transformed at a frequency of 3aEuroSxaEuroS10(-7) transformants per cfu, whereas no transconjugants were detected using S. pneumoniae or E. faecalis as recipient cells. Genome analysis showed that many other recombinations were scattered throughout the genome of the transformants in addition to transposon Tn2010. The transformants demonstrated a negligible fitness cost compared with the wild-type strain.

Tn2010 tended to be transferred by transformation rather than conjugation in S. pneumoniae, and the spread of Tn2010 could have a profound effect on the evolution of the genome. The acquisition of Tn2010 with negligible fitness cost may facilitate spread of the transposon.

语种英语
所属项目编号81071393 ; KSCX2-EW-J-6
资助者National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences
WOS记录号WOS:000336506300005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56982
专题北京大学第一临床医学院_临床药理研究所
作者单位1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China
2.Capital Med Univ, Beijing Pediat Res Inst, Beijing Childrens Hosp, Key Lab Major Dis Children,Minist Educ, Beijing, Peoples R China
3.Capital Med Univ, Beijing Pediat Res Inst, Beijing Childrens Hosp, Natl Key Discipline Pediat,Minist Educ, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100871, Peoples R China
5.Beijing Key Lab Microbial Drug Resistance & Resis, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Wenqing,Yao, Kaihu,Zhang, Gang,et al. Mechanism for transfer of transposon Tn2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,2014,69(6):1470-1473.
APA Zhou, Wenqing.,Yao, Kaihu.,Zhang, Gang.,Yang, Yonghong.,Li, Yun.,...&Feng, Jie.(2014).Mechanism for transfer of transposon Tn2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution.JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,69(6),1470-1473.
MLA Zhou, Wenqing,et al."Mechanism for transfer of transposon Tn2010 carrying macrolide resistance genes in Streptococcus pneumoniae and its effects on genome evolution".JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 69.6(2014):1470-1473.
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