北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 神经内科  > 期刊论文
学科主题: 临床医学
题名:
Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport
作者: Liu, XQ; Zhang, CG; Xing, GC; Chen, QT; He, FC
关键词: ARFGAP3 ; intracellular transport ; ADP-ribosylation factor (ARF) ; GTPase activating protein (GAP) ; biochemical activity
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2001-08-01
卷: 28, 期:4, 页:507-513
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: GTPASE-ACTIVATING PROTEIN ; ADP-RIBOSYLATION FACTOR ; CHOLERA-TOXIN ; BINDING ; COFACTOR
英文摘要:

ARF GAP Lis a kind of important regulator of introcellular transport. Recently, a novel human gene has been found from a cDNA library of second trimester human fetal liver. The amino acid sequence encoded by the novel gene has 32% similarity to rat ARF1 GAP, was thus termed as ARFGAP3. Functional studies of the new gene were performed. The full-length cDNA of ARFGAP3 was amplified from the human total placenta RNA by RT-PCR technique, then subcloned into pGEM-T vector and sequenced. The RNA Master blot and multiple tissue Northern blot analysis were used to define the expression profile and the transcript size of ARFGAP3 in human tissues. It was shown that ARFGAP3 was strongly expressed in glands and testis and that ARFGAP3 mRNA existed as only one kind of transcript of 2.7 kb in various human tissues. Then, the expression and purification of the recombinant human ARFGAP3 (rhARFGAP3) were performed. It was demonstrated that rhARFGAP3 exhibited strong GTPase-activating protein ( GAP) activity towards the recombinant ARF1 in vitro by an assay of a single round of GTP hydrolysis on recombinant ARF1, and that GAP activity of ARFGAP3 was stimulated by PIP2 and inhibited by PC.

语种: 中文
WOS记录号: WOS:000170807200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56985
Appears in Collections:北京大学第一临床医学院_神经内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Acad Mil Med Sci, Inst Radiat Med, Beijing 100850, Peoples R China
2.Beijing Med Univ, Dept Neurol, Teaching Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Liu, XQ,Zhang, CG,Xing, GC,et al. Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2001,28(4):507-513.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, XQ]'s Articles
[Zhang, CG]'s Articles
[Xing, GC]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, XQ]‘s Articles
[Zhang, CG]‘s Articles
[Xing, GC]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace