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Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport
Liu, XQ; Zhang, CG; Xing, GC; Chen, QT; He, FC
关键词Arfgap3 Intracellular Transport Adp-ribosylation Factor (Arf) Gtpase Activating Protein (Gap) Biochemical Activity
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2001-08-01
28期:4页:507-513
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GTPASE-ACTIVATING PROTEIN ; ADP-RIBOSYLATION FACTOR ; CHOLERA-TOXIN ; BINDING ; COFACTOR
英文摘要

ARF GAP Lis a kind of important regulator of introcellular transport. Recently, a novel human gene has been found from a cDNA library of second trimester human fetal liver. The amino acid sequence encoded by the novel gene has 32% similarity to rat ARF1 GAP, was thus termed as ARFGAP3. Functional studies of the new gene were performed. The full-length cDNA of ARFGAP3 was amplified from the human total placenta RNA by RT-PCR technique, then subcloned into pGEM-T vector and sequenced. The RNA Master blot and multiple tissue Northern blot analysis were used to define the expression profile and the transcript size of ARFGAP3 in human tissues. It was shown that ARFGAP3 was strongly expressed in glands and testis and that ARFGAP3 mRNA existed as only one kind of transcript of 2.7 kb in various human tissues. Then, the expression and purification of the recombinant human ARFGAP3 (rhARFGAP3) were performed. It was demonstrated that rhARFGAP3 exhibited strong GTPase-activating protein ( GAP) activity towards the recombinant ARF1 in vitro by an assay of a single round of GTP hydrolysis on recombinant ARF1, and that GAP activity of ARFGAP3 was stimulated by PIP2 and inhibited by PC.

语种中文
WOS记录号WOS:000170807200017
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56985
专题北京大学第一临床医学院_神经内科
作者单位1.Acad Mil Med Sci, Inst Radiat Med, Beijing 100850, Peoples R China
2.Beijing Med Univ, Dept Neurol, Teaching Hosp 1, Beijing 100034, Peoples R China
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GB/T 7714
Liu, XQ,Zhang, CG,Xing, GC,et al. Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2001,28(4):507-513.
APA Liu, XQ,Zhang, CG,Xing, GC,Chen, QT,&He, FC.(2001).Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,28(4),507-513.
MLA Liu, XQ,et al."Cloning, expression and biochemical activity of ARFGAP3, a regulator of intracellular transport".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 28.4(2001):507-513.
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