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学科主题: 临床医学
题名:
Inhibitory effect of human interferon-beta-1a on activated rat and human hepatic stellate cells
作者: Rao, Hui-Ying; Wei, Lai; Wang, Jiang-Hua; Fei, Ran; Jiang, Dong; Zhang, Quan; Chen, Hong-Song; Cong, Xu
关键词: hepatic stellate cell ; interferon-beta ; platelet-derived growth factor ; transforming growth factor-beta
刊名: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
发表日期: 2010-11-01
DOI: 10.1111/j.1440-1746.2010.06264.x
卷: 25, 期:11, 页:1777-1784
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: GROWTH-FACTOR-BETA ; LIVER FIBROSIS ; HUMAN-DISEASE ; TGF-BETA ; ALPHA ; PROLIFERATION ; MECHANISMS ; CIRRHOSIS ; THERAPY ; TARGETS
英文摘要:

Background and Aims:

Hepatic stellate cells (HSC) are the primary cell type mediating hepatic fibrosis. Although known for its antiviral effects, the inhibitory effects of interferon-beta (IFN-beta) on HSC treatment have not yet been established.

Methods:

Both human and rat activated HSC cell lines were incubated with increasing concentrations of recombinant human IFN-beta 1a (rhIFN-beta 1a) for 24, 48 or 72 h. The effects of rhIFN-beta 1a on alpha-smooth muscle actin (alpha-SMA), collagen types I and III, transforming growth factor-beta 1 (TGF-beta 1), platelet-derived growth factor-BB (PDGF-BB), and mothers against decapentaplegic homolog (Smad4, Smad7) expression in HSC were examined using Western blotting and immunocytochemistry. Proliferation of HSC was evaluated via bromodeoxyuridine assay.

Results:

rhIFN-beta 1a treatment had a dose-dependent, inhibitory effect on alpha-SMA and collagen type I protein expression. In addition, rhIFN-beta 1a decreased the expression of collagen type III, TGF-beta 1, PDGF-BB and Smad4 protein expression in HSC compared with untreated cells. We also observed increased Smad7 protein expression and decreased proliferation in rhIFN-beta 1a-treated HSC.

Conclusions:

Our data suggest that rhIFN-beta 1a treatment decreased alpha-SMA and collagen expression and inhibited the activation of HSC through the inhibition of the TGF-beta and PDGF pathways.

语种: 英语
WOS记录号: WOS:000283597900013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57000
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Rao, Hui-Ying,Wei, Lai,Wang, Jiang-Hua,et al. Inhibitory effect of human interferon-beta-1a on activated rat and human hepatic stellate cells[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2010,25(11):1777-1784.
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