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Screening the bioactive compounds in aqueous extract of Coptidis rhizoma which specifically bind to rabbit lung tissues beta(2)-adrenoceptor using an affinity chromatographic selection method
Zhao, Xinfeng1; Nan, Yefei1; Xiao, Chaoni1; Zheng, Jianbin2; Zheng, Xiaohui1; Wei, Yinmao2; Zhang, Youyi3,4
关键词Affinity Chromatography Beta(2)-adrenoceptor Coptidis Rhizome Bioactive Compound Column Switching
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2010-07-15
DOI10.1016/j.jchromb.2010.05.040
878期:22页:2029-2034
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BETA-ADRENERGIC-RECEPTOR ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINES ; TANDEM MASS-SPECTROMETRY ; DRUG DISCOVERY ; STATIONARY-PHASE ; NATURAL-PRODUCTS ; NONCOMPETITIVE INHIBITORS ; ANGELICA-SINENSIS ; BLOOD-PRESSURE
英文摘要

A receptor affinity chromatographic selection method was developed for screening the bioactive compounds binding to beta(2)-adrenoceptor (beta(2)-AR) in Coptidis rhizome. The bioactive compounds were analyzed by molecular recognition with a beta(2)-AR affinity column. The retention compounds eluted from the beta(2)-AR column were separated online with reverse-phase high-performance liquid chromatography by column switching technology, and identified by a coupled ion-trap mass spectrometer. Four compounds were screened as the bioactive compounds of Coptidis rhizome and identified as 2,9,10-trimethoxy-3-hydroxyl-protoberberine (jateorhizine), 2,3-methylenedioxy-9-methoxy-protoberberine, 2,3,9,10-tetramethoxy-protoberberine (palmatine) and 2,3-methylenedioxy-9,10-dimethoxy-protoberberine (berberine). The association constants of jatrorrhizine, palmatine and berberine to the beta(2)-AR were determined by the zonal elution method with standards. Berberine and palmatine had only one type of binding site on the immobilized beta(2)-AR. Their association constants were (2.28 +/- 0.11) x 10(4)/M and (3.00 +/- 0.10) x 10(4)/M, respectively. Jatrorrhizine had at least two type of binding sites on the immobilized beta(2)-AR, and the corresponding association constants were (2.20 +/- 0.09) x 10(-4)/M and (6.78 +/- 0.001) x 10(5)/M. (C) 2010 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000280210300018
项目编号20875074 ; 20875075 ; 2007DF02 ; 207151
资助机构National Natural Sciences Foundation of China ; West Light Foundation of The Chinese Academy of Sciences ; Ministry of Education
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57003
专题北京大学第三临床医学院_心血管内科
作者单位1.NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China
2.NW Univ Xian, Inst Analyt Sci, Xian 710069, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China
4.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
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GB/T 7714
Zhao, Xinfeng,Nan, Yefei,Xiao, Chaoni,et al. Screening the bioactive compounds in aqueous extract of Coptidis rhizoma which specifically bind to rabbit lung tissues beta(2)-adrenoceptor using an affinity chromatographic selection method[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(22):2029-2034.
APA Zhao, Xinfeng.,Nan, Yefei.,Xiao, Chaoni.,Zheng, Jianbin.,Zheng, Xiaohui.,...&Zhang, Youyi.(2010).Screening the bioactive compounds in aqueous extract of Coptidis rhizoma which specifically bind to rabbit lung tissues beta(2)-adrenoceptor using an affinity chromatographic selection method.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(22),2029-2034.
MLA Zhao, Xinfeng,et al."Screening the bioactive compounds in aqueous extract of Coptidis rhizoma which specifically bind to rabbit lung tissues beta(2)-adrenoceptor using an affinity chromatographic selection method".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.22(2010):2029-2034.
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