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学科主题: 基础医学
题名:
EGF Protects Cells Against Dox-Induced Growth Arrest Through Activating Cyclin D1 Expression
作者: Yao, Chun-Xia1; Shi, Jia-Chen1; Ma, Cai-Xia1; Xiong, Cheng-Juan1; Song, Yang-Liu1; Zhang, Shu-Feng2; Zhang, Shan-Feng1; Zang, Ming-Xi1; Xue, Li-Xiang3
关键词: CELL GROWTH ; DOX ; EGF ; GATA-4 ; CYCLIN D1
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2015-08-01
DOI: 10.1002/jcb.25134
卷: 116, 期:8, 页:1755-1765
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: GENE-EXPRESSION ; CARDIOMYOCYTE PROLIFERATION ; IN-VITRO ; DIFFERENTIATION ; DOXORUBICIN ; GATA-4 ; CARCINOMA ; APOPTOSIS ; PATHWAY ; VIVO
英文摘要:

It has been reported that the antitumor drug doxorubicin (Dox) exerts its toxic effects via GATA-4 depletion and that over-expression of GATA-4 reverses Dox-induced toxicity and apoptosis; however, the precise mechanisms remain unclear. In this study, we observed, for the first time, that EGF protects cells against Dox-mediated growth arrest, G2/M-phase arrest, and apoptosis. Additionally, EGF expression was down-regulated in Dox-treated cells and up-regulated in GATA-4 over-expressing cells. Utilizing real-time PCR and western blotting analysis, we found that the expression of the cell cycle-associated protein cyclin D1 was inhibited in GATA-4-silenced cells and Dox-treated cells and was enhanced in GATA-4 over-expressing cells and EGF-treated cells. Furthermore, EGF treatment reversed the inhibited expression of cyclin D1 that was mediated by GATA-4 RNAi or Dox. Our results indicate that EGF, as a downstream target of Dox, may be involved in Dox-induced toxicity as well as in the protective role of GATA-4 against toxicity induced by Dox via regulating cyclin D1 expression, which elucidates a new molecular mechanism of Dox toxicity with important clinical implications. J. Cell. Biochem. 116: 1755-1765, 2015. (c) 2015 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 81371895 ; 81141044 ; 31071206 ; 2012AA022501
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000356525800026
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57007
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Zhengzhou Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Zhengzhou 450001, Henan, Peoples R China
2.Zhengzhou Univ, Peoples Hosp Henan Prov, Zhengzhou 450001, Henan, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China

Recommended Citation:
Yao, Chun-Xia,Shi, Jia-Chen,Ma, Cai-Xia,et al. EGF Protects Cells Against Dox-Induced Growth Arrest Through Activating Cyclin D1 Expression[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2015,116(8):1755-1765.
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