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学科主题: 基础医学
题名:
PLD1 Negatively Regulates Dendritic Branching
作者: Zhu, Yan-Bing1,2; Kang, Kai1,2; Zhang, Ying1,2; Qi, Cai1,2; Li, Gang1,2; Yin, Dong-Min3; Wang, Yun1,2
刊名: JOURNAL OF NEUROSCIENCE
发表日期: 2012-06-06
DOI: 10.1523/JNEUROSCI.5378-11.2012
卷: 32, 期:23, 页:7960-7969
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE ; PHOSPHOLIPASE-D ISOFORMS ; PROTEIN-KINASE-C ; ADP-RIBOSYLATION FACTOR ; RHO-ASSOCIATED KINASE ; PHOSPHATIDIC-ACID ; NEURITE OUTGROWTH ; INCREASED EXPRESSION ; HIPPOCAMPAL-NEURONS ; ACTIN CYTOSKELETON
英文摘要:

Neurons have characteristic dendritic arborization patterns that contribute to information processing. One essential component of dendritic arborization is the formation of a specific number of branches. Although intracellular pathways promoting dendritic growth and branching are being elucidated, the mechanisms that negatively regulate the branching of dendrites remain enigmatic. In this study, using gain-of-function and loss-of-function studies, we show that phospholipase D1 (PLD1) acts as a negative regulator of dendritic branching in cultured hippocampal neurons from embryonic day 18 rat embryos. Overexpression of wild-type PLD1 (WT-PLD1) decreases the complexity of dendrites, whereas knockdown or inhibition of PLD1 increases dendritic branching. We further demonstrated that PLD1 acts downstream of RhoA, one of the small Rho GTPases, to suppress dendritic branching. The restriction of dendritic branching by constitutively active RhoA (V14-RhoA) can be partially rescued by knockdown of PLD1. Moreover, the inhibition of dendritic branching by V14-RhoA and WT-PLD1 can be partially ameliorated by reducing the level of phosphatidic acid (PA), which is the enzymatic product of PLD1. Together, these results suggest that RhoA-PLD1-PA may represent a novel signaling pathway in the restriction of dendritic branching and may thus provide insight into the mechanisms of dendritic morphogenesis.

语种: 英语
所属项目编号: 81161120497 ; 30830044 ; 30900582 ; 30925015 ; 7092061 ; 2007CB512100 ; 200800011028 ; 20060001121
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research 973 Program of China ; Specialized Research Fund for Doctoral Program of Higher Education
WOS记录号: WOS:000305091800020
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57035
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Key Lab Neurosci, Minist Educ & Hlth,Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Georgia Hlth Sci Univ, Inst Mol Med & Genet, Augusta, GA 30912 USA

Recommended Citation:
Zhu, Yan-Bing,Kang, Kai,Zhang, Ying,et al. PLD1 Negatively Regulates Dendritic Branching[J]. JOURNAL OF NEUROSCIENCE,2012,32(23):7960-7969.
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