北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔修复科  > 期刊论文
学科主题: 口腔医学
题名:
Fluocinolone Acetonide Promotes the Proliferation and Mineralization of Dental Pulp Cells
作者: Liu, Zhongning1; Jiang, Ting1; Wang, Yixiang2; Wang, Xinzhi1
关键词: Dental pulp cells ; fluocinolone acetonide ; mineralization ; proliferation
刊名: JOURNAL OF ENDODONTICS
发表日期: 2013-02-01
DOI: 10.1016/j.joen.2012.09.012
卷: 39, 期:2, 页:217-222
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: ORAL LICHEN-PLANUS ; MESENCHYMAL STEM-CELLS ; IFN-GAMMA ; TNF-ALPHA ; IN-VITRO ; SIALOPHOSPHOPROTEIN ; REGENERATION ; EXPRESSION ; 0.1-PERCENT ; TISSUES
英文摘要:

Introduction: The aim of this study was to investigate the role of the steroid fluocinolone acetonide on the proliferation and mineralization of human dental pulp cells (DPCs). The potential effect of fluocinolone acetonide on reparative dentin formation and the recovery of injured dental pulp were evaluated. Methods: The proliferative effect of fluocinolone acetonide on DPCs was analyzed by cholecystokinin octapeptide assay and flow cytometry. The mineralized effect of fluocinolone acetonide was investigated by the detection of mineralization-related biomarkers including alkaline phosphatase (ALP), bone sialoprotein, and osteocalcin by using ALP histochemical staining, ALP activity, immunostaining, alizarin red staining, and reverse-transcriptase polymerase chain reaction. The molecules, including dentin sialophosphoprotein and Wnt4, involved in the process of mineralization were detected by real-time polymerase chain reaction and Western blot analysis. Results: Low concentrations of fluocinolone acetonide (0.1-40 mu mol/L) promoted the proliferation of DPCs. The flow cytometry results showed that the CD146-positive subpopulation of DPCs was significantly increased after treatment with fluocinolone acetonide at 1 and 10 mu mol/L for 48 hours, respectively. The messenger RNA expression and activity of the early-stage mineralization marker ALP were evidently increased in fluocinolone acetonide treated DPCs compared with the untreated control group, so did the middle-stage mineralization marker bone sialoprotein and the late-stage mineralization marker osteocalcin. Meanwhile, Wnt4 and the dentin-specific marker dentin sialophosphoprotein were obviously up-regulated by fluocinolone acetonide compared with the untreated controls. Conclusions: Fluocinolone acetonide can promote the proliferation of DPCs, especially for the CD146+ subpopulation. Fluocinolone acetonide can initiate the mineralization of DPCs and has the potential role in repairing injured pulp tissues. (J Endod 2013;39:217-222)

语种: 英语
所属项目编号: 30973353
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000314482500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57047
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Zhongning,Jiang, Ting,Wang, Yixiang,et al. Fluocinolone Acetonide Promotes the Proliferation and Mineralization of Dental Pulp Cells[J]. JOURNAL OF ENDODONTICS,2013,39(2):217-222.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Zhongning]'s Articles
[Jiang, Ting]'s Articles
[Wang, Yixiang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Zhongning]‘s Articles
[Jiang, Ting]‘s Articles
[Wang, Yixiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace