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学科主题: 临床医学
题名:
Nongenomic Effect of Estrogen on the MAPK Signaling Pathway and Calcium Influx in Endometrial Carcinoma Cells
作者: Zhang, Lili; Li, Xiaoping; Zhao, Lijun; Zhang, Lifang; Zhang, Guo; Wang, Jianliu; Wei, Lihui
关键词: NONGENOMIC EFFECT ; ENDOMETRIAL CARCINOMA ; Erk1/2 ; Akt ; CALCIUM ; BCL-2
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2009-03-01
DOI: 10.1002/jcb.22017
卷: 106, 期:4, 页:553-562
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: RAT HIPPOCAMPAL-NEURONS ; PROTEIN-KINASE-A ; ADENOCARCINOMA CELLS ; ENDOTHELIAL-CELLS ; TRAUMA-HEMORRHAGE ; BREAST-CANCER ; SMOOTH-MUSCLE ; UP-REGULATION ; MCF-7 CELLS ; RECEPTOR
英文摘要:

17 beta-Estradiol (E2) is well known to interact with intracelluar receptors that act as nuclear transcription factors. However, abundant evidence now indicates that E2 can also rapidly induce several nongenomic effects through signaling pathways related to cell growth, preservation, and differentiation. We studied the nongenomic effects of E2 in two human endometrial carcinoma cell lines, Ishikawa (estrogen receptor (ER) positive) and Hec-1A (ER negative or low) by cultivating them with either E2 or its membrane-impermeable conjugate, E2-BSA. We found that phosphorylalion of Erk1/2 could he induced by either E2 or E2-BSA in Ishikawa cells. In Hec-1A cells, only E2 was able to induce Erk1/2 phosphorylation. Although the existence or a nongenomic component to the response was indicated by the finding that it could not be completely inhibited by the ER antagonist ICI 182780,and it can also be inhibited by calcium inhibitor Nifedipine partly. Phosphorylation of Akt could not be induced, either by E2. or E2-BSA, in either cell line. Both E2 and E2-BSA elicited calcium influx in Ishikawa cells. In contrast to these nongenomic effects, only E2 was able to Stimulate expression of the anti-apoptotic-protein Bcl-2. Taken together, these data indicate that nongenomic effects such as Erk1/2 phosphorylation and calcium influx can be initiated from the membrane in Ishikawa cell, and calcium can activate Erk1/2 phosphorylation. Except for ER, there must be other binding location of estrogen in endometrial cancer cells, and the nongenomic effects of estrogen initiated from plasma membrane fly E2-BSA cannot lead to transcriptional effect of Bcl-2 expression. J. Cell. Biochem. 106: 553-562, 2009. (C) 2009 Wiley-Liss, Inc.

语种: 英语
所属项目编号: 30571937
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000264456300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57051
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Lili,Li, Xiaoping,Zhao, Lijun,et al. Nongenomic Effect of Estrogen on the MAPK Signaling Pathway and Calcium Influx in Endometrial Carcinoma Cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2009,106(4):553-562.
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