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学科主题: 药学
题名:
A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats
作者: Yang, Hao1; Wang, Guangji1; Hao, Haiping1; Tu, Pengfei2; Jiang, Yong2; Wang, Qiong1; Zhang, Yan1; Zheng, Chaonan1; Wanga, Yuxin1; Dai, Liang1
关键词: echinacoside ; LC/MS/MS ; pharmacokinetics ; phenylethanoid glycoside ; antioxidant
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2009-06-01
DOI: 10.1002/bmc.1164
卷: 23, 期:6, 页:630-637
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PHENOLIC-COMPOUNDS ; ANTIOXIDANT ; BIOAVAILABILITY ; PLANT ; HPLC
英文摘要:

A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid-liquid extraction with n-butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell-MG C(18) analytical column (100 x 2.0 mm x 5 mu m), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10-2500 ng/mL. The deviations of both intra- and inter-day precisions (RSD) were 7.1% and the assay accuracies were within 99.2-106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 30572228 ; 30630076 ; 2006BAI08B04-05
项目资助者: National Natural Science Foundation of P.R. China ; Ministry of Science and Technology of P.R. China
WOS记录号: WOS:000266182600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57053
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing 210038, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Yang, Hao,Wang, Guangji,Hao, Haiping,et al. A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats[J]. BIOMEDICAL CHROMATOGRAPHY,2009,23(6):630-637.
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