IR@PKUHSC  > 北京大学药学院  > 天然药物学系
学科主题药学
A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats
Yang, Hao1; Wang, Guangji1; Hao, Haiping1; Tu, Pengfei2; Jiang, Yong2; Wang, Qiong1; Zhang, Yan1; Zheng, Chaonan1; Wanga, Yuxin1; Dai, Liang1
关键词Echinacoside Lc/ms/ms Pharmacokinetics Phenylethanoid Glycoside Antioxidant
刊名BIOMEDICAL CHROMATOGRAPHY
2009-06-01
DOI10.1002/bmc.1164
23期:6页:630-637
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PHENOLIC-COMPOUNDS ; ANTIOXIDANT ; BIOAVAILABILITY ; PLANT ; HPLC
英文摘要

A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid-liquid extraction with n-butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell-MG C(18) analytical column (100 x 2.0 mm x 5 mu m), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10-2500 ng/mL. The deviations of both intra- and inter-day precisions (RSD) were 7.1% and the assay accuracies were within 99.2-106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000266182600010
项目编号30572228 ; 30630076 ; 2006BAI08B04-05
资助机构National Natural Science Foundation of P.R. China ; Ministry of Science and Technology of P.R. China
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57053
专题北京大学药学院_天然药物学系
通讯作者Wang, Guangji
作者单位1.China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing 210038, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yang, Hao,Wang, Guangji,Hao, Haiping,et al. A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats[J]. BIOMEDICAL CHROMATOGRAPHY,2009,23(6):630-637.
APA Yang, Hao.,Wang, Guangji.,Hao, Haiping.,Tu, Pengfei.,Jiang, Yong.,...&Dai, Liang.(2009).A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats.BIOMEDICAL CHROMATOGRAPHY,23(6),630-637.
MLA Yang, Hao,et al."A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats".BIOMEDICAL CHROMATOGRAPHY 23.6(2009):630-637.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Hao]的文章
[Wang, Guangji]的文章
[Hao, Haiping]的文章
百度学术
百度学术中相似的文章
[Yang, Hao]的文章
[Wang, Guangji]的文章
[Hao, Haiping]的文章
必应学术
必应学术中相似的文章
[Yang, Hao]的文章
[Wang, Guangji]的文章
[Hao, Haiping]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。