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学科主题: 公共卫生
题名:
Tumor targeting of I-125-labeled anti-EGFR monoclonal antibody LA22 in HT-29 human colon cancer
作者: Zhao Huiyun; Jia Bing; Wang Fan; Liu Zhaofei1
关键词: Epidermal growth factor receptor ; LA22 ; Monoclonal antibody ; Tumor targeting ; Gamma imaging
刊名: NUCLEAR SCIENCE AND TECHNIQUES
发表日期: 2010-04-20
卷: 21, 期:2, 页:84-88
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nuclear Science & Technology ; Physics, Nuclear
研究领域[WOS]: Nuclear Science & Technology ; Physics
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ; FACTOR RECEPTOR ; BINDING
英文摘要:

Epidermal growth factor receptor (EGER) plays a critical role in proliferation, apoptosis, angiogenesis, invasiveness and distant metastasis of tumors. In this study, the tumor targeting properties of anti-EGFR monoclonal antibody (mAb) LA22 in a colon cancer mouse model are evaluated. The results from flow cytometry assay and immunofluorescent staining clearly showed that HT-29 human colon cancer cells were EGFR positive, and the binding of mAb LA22 to the HT-29 cell surface was specific. The saturation binding experiment of I-125-LA22 to HT-29 cells revealed that LA22 possessed moderate affinity to EGFR with the Kd value calculated to be 3.28 +/- 0.76 nM. The in vivo gamma imaging demonstrated the specific accumulation of I-125-LA22 in HT-29 tumor xenografts. The specific tumor targeting properties of mAb LA22 make it a good candidate for tumor targeted radioimmunotherapy of EGFR-positive tumors when it is labeled with therapeutic nuclides, such as I-131, Lu-177, or Y-90.

语种: 英语
所属项目编号: 2007AA02Z467 ; 30640067 ; 30870728
项目资助者: Chinese National "863" project ; NSF
WOS记录号: WOS:000278639500006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57054
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Dept Radiat Med, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zhao Huiyun,Jia Bing,Wang Fan,et al. Tumor targeting of I-125-labeled anti-EGFR monoclonal antibody LA22 in HT-29 human colon cancer[J]. NUCLEAR SCIENCE AND TECHNIQUES,2010,21(2):84-88.
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