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学科主题: 基础医学
题名:
Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line
作者: Li, Wei-dong1; Zhang, Bo-di1; Wei, Ran1; Liu, Ji-hong2; Lin, Zhi-bin1
关键词: multidrug resistance ; Ganoderma lucidum polysaccharides ; K562/adriamycin ; P-glycoprotein ; multidrug resistance-1 ; multidrug resistance-associated protein-1
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2008-05-01
DOI: 10.1111/j.1745-7254.2008.00776.x
卷: 29, 期:5, 页:620-627
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: GENE-EXPRESSION ; MDR1 GENE ; ANTITUMOR ; BREAST ; VINBLASTINE ; MODULATION ; PEPTIDE
英文摘要:

Aim: To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM. Methods: Cytotoxicity was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR Results: Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP 1 transcription were downregulated by 10 and 50 mg/L Gl-PS. Conclusion: Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.

语种: 英语
WOS记录号: WOS:000255419700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57075
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Confocal Microscope Lab, Beijing 100083, Peoples R China

Recommended Citation:
Li, Wei-dong,Zhang, Bo-di,Wei, Ran,et al. Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line[J]. ACTA PHARMACOLOGICA SINICA,2008,29(5):620-627.
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