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Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells
Jin, Hong-Fang; Du, Jun-Bao; Li, Xiao-Hui; Wang, Yan-Fei; Liang, Yin-Fang; Tang, Chao-Shu
关键词Hydrogen Sulfide Cystathionine Gamma-lyase Carbon Monoxide Heme Oxygenase
刊名ACTA PHARMACOLOGICA SINICA
2006-12-01
DOI10.1111/j.1745-7245.2006.00425.x
27期:12页:1561-1566
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]NITRIC-OXIDE ; SULFIDE ; PROLIFERATION ; RAT ; HYPERTENSION ; PATHOGENESIS ; MEDICINE ; BIOLOGY ; H2S
英文摘要

Aim: To investigate the interaction between hydrogen sulfide (H2S)/cystathion-ine gamma-lyase (CSE) and carbon monoxide (CO)/heme oxygenase (HO) pathways in aortic smooth muscle cells (ASMC). Methods: The ASMCs were divided into the following groups: (1) the control group; (2) the zinc protoporphyrin (ZnPP) 20 mu mol/L group; (3) the propargylglycine (PPG) 2mmol/L, 4 mmol/L and 10 mmol/L groups; and (4) the sodium hydrosulfide (NaHS) 1 x 10(-5) mol/L, 1 x 10(-4) mol/L and 1 x 10(-3) mol/L groups. Each of the groups was further divided into 6 h, 12 h, 18 h and 24 h subgroups. The CO level, represented by carboxyhemoglobin (HbCO) content was measured using a spectrophotometric method and H2S content was detected by a sensitive electrode method. CSE and HO-1 expressions were detected by Western blotting. Results: The H2S content in the medium and CSE expression by ASMC were markedly increased by ZnPP compared with the control group. HbCO content in the medium and HO-1 expression by the ASMC started strengthening following 24 h treatment with PPG at 2 mmol/L, but were further strengthened following 18 h and 24 h treatment with PPG at 4 mmol/L compared with the controls (P < 0.01). PPG at 10 mmol/L increased the HbCO level in the medium following 18 h treatment and increased HO-1 expression by the ASMC following 12 h treatment. Moreover, NaHS at 1 x 10(-5) mol/L and 1 x 10(-4) mol/L decreased the HbCO level in the medium and HO-1 expression by the ASMC after 6 h and 12 h treatment, while NaHS at 1 x 10(-3) mol/L decreased them at all time points of the treatments. Conclusion: The results suggested that endogenous CO/HO and H2S/CSE pathways inhibited each other in ASMC under physiological conditions.

语种英语
WOS记录号WOS:000242221300006
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57091
专题北京大学第一临床医学院_儿科
作者单位1.Peking Univ, First Hosp, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Inst Cardiovasc Res, First Hosp, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Jin, Hong-Fang,Du, Jun-Bao,Li, Xiao-Hui,et al. Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells[J]. ACTA PHARMACOLOGICA SINICA,2006,27(12):1561-1566.
APA Jin, Hong-Fang,Du, Jun-Bao,Li, Xiao-Hui,Wang, Yan-Fei,Liang, Yin-Fang,&Tang, Chao-Shu.(2006).Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells.ACTA PHARMACOLOGICA SINICA,27(12),1561-1566.
MLA Jin, Hong-Fang,et al."Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells".ACTA PHARMACOLOGICA SINICA 27.12(2006):1561-1566.
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