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学科主题: 口腔医学
题名:
Iron plays a key role in the cytodifferentiation of human periodontal ligament cells
作者: Hou, J.1,2; Yamada, S.2; Kajikawa, T.2; Ozaki, N.2; Awata, T.2; Yamaba, S.2; Fujihara, C.2; Murakami, S.2
关键词: periodontal ligament cells ; human ; iron ; cytodifferentiation
刊名: JOURNAL OF PERIODONTAL RESEARCH
发表日期: 2014-04-01
DOI: 10.1111/jre.12103
卷: 49, 期:2, 页:260-267
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: BONE-MINERAL DENSITY ; POSTMENOPAUSAL WOMEN ; DIETARY IRON ; STEM-CELLS ; RATS ; FERRITIN ; OSTEOMALACIA ; MECHANISMS ; CHELATORS ; PROTEINS
英文摘要:

Background and Objective

The periodontal ligament (PDL) is vital to maintaining the homeostasis of the tooth and periodontal tissue. The influence of iron levels on the cytodifferentiation of PDL cells has not been studied, despite evidence that iron overload or deficiency can have adverse effects on alveolar bone density. The purpose of this study was to examine the effects of altered iron levels on cytodifferentiation in human PDL cells.

Material and Methods

Human PDL cells were incubated with culture media supplemented with 10-50 mu m ammonium ferric citrate or 5 mu m deferoxamine (an iron chelator) during differentiation. Intracellular iron status was assessed by measuring changes in the expression of ferritin RNA and protein. PDL cell differentiation and function were evaluated by measuring osteoblast differentiation gene markers and the capacity of cultures to form mineralized nodules.

Results

Iron accumulation resulted in upregulation of light and heavy chain ferritin proteins. Concurrently, osteoblast differentiation gene markers and mineralized nodule formation were suppressed. Iron deficiency resulted in downregulation of light and heavy chain ferritin proteins, suppression of alkaline phosphatase activity and formation of mineralized nodules during PDL cell differentiation.

Conclusion

We conclude that iron is critical for normal cell differentiation of human PDL cells.

语种: 英语
所属项目编号: 23249086 ; 23390478
项目资助者: Japan Society for the Promotion of Science ; China Scholarship Council (CSC)
WOS记录号: WOS:000332773300014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57098
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China
2.Osaka Univ, Grad Sch Dent, Dept Periodontol, Suita, Osaka 5650871, Japan

Recommended Citation:
Hou, J.,Yamada, S.,Kajikawa, T.,et al. Iron plays a key role in the cytodifferentiation of human periodontal ligament cells[J]. JOURNAL OF PERIODONTAL RESEARCH,2014,49(2):260-267.
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