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学科主题: 临床医学
题名:
Loss of Expression of Growth Differentiation Factor-9 (GDF9) in Human Kidney Cancer and Regulation of Growth and Migration of Kidney Cancer Cells by GDF9
作者: Du, Peng1,2,3; Ye, Lin2,3; Li, Han4; Ruge, Fiona2,3; Yang, Yong1; Jiang, Wen G.2,3
关键词: Growth differentiation factor-9 ; GDF9 ; cellular adhesion ; migration ; invasion ; growth ; kidney cancer ; UMRC-2 ; CAKI-2 cells
刊名: ANTICANCER RESEARCH
发表日期: 2012-10-01
卷: 32, 期:10, 页:4375-4383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: HUMAN BREAST-CANCER ; IN-VITRO ; MEMBRANE ; IMPACT ; FOLLICULOGENESIS ; INHIBITION ; PROMOTES ; MOTILITY ; OOCYTES ; GENE
英文摘要:

Background: Growth differentiation factor-9 (GDF9), a member of the bone morphogenetic protein (BMP) family and the transforming growth factor (TGF)-beta superfamily, has recently been implicated in the biological control of cancer cell behaviour. It has also been implicated in the development and spread of solid cancer. However, the role of GDF9 in kidney cancer remains to be investigated. In the present study, the expression of GDF9 in normal and malignant human kidney tissues and its molecular and cellular impact on human kidney cancer cells were investigated. Materials and Methods: The expression of GDF9 in human kidney tissues and kidney cancer cell lines (UMRC-2 and CAKI-2) was assessed at both the mRNA and protein levels using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. GDF9 overexpression was induced by a mammalian GDF9 expression construct. The effect of GDF9 expression on cellular functions was examined in kidney cancer cells overexpressing GDF9 using a variety of in vitro assays. Results: In normal kidney tissues, stronger staining of GDF9 was seen in renal tubular epithelial cells, both in the cytoplasm and in the nucleus. In contrast, the staining of GDF9 was notably weak or absent in cells of tumour tissues. Human kidney cancer cell lines UMRC-2 and CAKI-2 had lost their GDG-9 expression. Overexpression of GDF9 reduced in vitro invasion,and cellular growth and migration of kidney cell lines in vitro. Using the electric cell-substrate sensing (ECIS) method, it was further revealed that overexpression of GDF9 in these cells markedly reduced cellular migration and adhesion. Conclusion: Human kidney tumours have a reduced or loss of expression of GDF9. In vitro, GDF9 overexpression suppresses the invasiveness, growth and migration of kidney cancer cells. This suggests that GDF9 is a potential tumour suppressor and may have prognostic and therapeutic implications in human kidney cancer.

语种: 英语
项目资助者: Albert Hung Foundation ; Cancer Research Wales ; Cardiff University China Medical Scholarship
WOS记录号: WOS:000310111800025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57110
Appears in Collections:北京大学临床肿瘤学院_泌尿外科_期刊论文

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作者单位: 1.Cardiff Univ Peking Univ Sch Oncol Joint Inst, Cardiff, S Glam, Wales
2.Peking Univ, Canc Hosp, Dept Urol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
3.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff, S Glam, Wales
4.Capital Med Univ, Chaoyang Hosp, Dept Urol, Beijing, Peoples R China

Recommended Citation:
Du, Peng,Ye, Lin,Li, Han,et al. Loss of Expression of Growth Differentiation Factor-9 (GDF9) in Human Kidney Cancer and Regulation of Growth and Migration of Kidney Cancer Cells by GDF9[J]. ANTICANCER RESEARCH,2012,32(10):4375-4383.
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