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学科主题: 基础医学
题名:
Oxidant stress mechanism of homocysteine potentiating Con A-induced proliferation in murine splenic T lymphocytes
作者: Zhang, Q; Zeng, XK; Guo, JX; Wang, M
关键词: apoptosis ; atherosclerosis ; free radicals ; immunology ; infection/inflammation ; leukocytes
刊名: CARDIOVASCULAR RESEARCH
发表日期: 2002-03-01
卷: 53, 期:4, 页:1035-1042
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: HUMAN ATHEROSCLEROTIC PLAQUES ; E-KNOCKOUT MICE ; SIGNAL-TRANSDUCTION ; HYDROGEN-PEROXIDE ; RISK FACTOR ; CELLS ; RECEPTOR ; GROWTH ; GENERATION ; ACTIVATION
英文摘要:

Objective: An elevated plasma homocysteine (Hey) level is considered an independent risk factor for atherosclerosis. However, the mechanisms by which hyperhomocysteinemia induces atherosclerosis are only partially understood. The effect of Hey on T lymphocyte proliferation and its mechanisms were examined in normal and hyperhomocysteinemia ApoE-knockout mice. Methods: The mouse splenic T-cells were treated with Hey, related compounds and/or antioxidants in the presence or absence of Concanavalin A (Con A). DNA synthesis, cell apoptosis, interleukin-2 level and production of reactive oxygen species (ROS) were measured. Results: Hey (0.3-3.0 mM) and related compounds with thiol (-SH), such as cysteine and glutathione significantly potentiated Con A-induced proliferation and partially inhibited apoptosis in T lymphocytes, but it had no direct effect on resting T lymphocyte. ApoE-knockout mice with hyperhomocysteinemia (the level of plasma Hey was 20.3+/-2.9 vs. 2.6+/-0.6 muM in control group, P<0.05) had a significant promotion of T-cell proliferation in response to Con A. Hey (0.3-3.0 mM) also increased the intracellular ROS. Radical scavengers reduced Hey effect. Conclusions: These data indicate that ROS generated by thiol (-SH) of Hey auto-oxidation are involved in Hey effect on Con A-induced T lymphocyte proliferation. These findings suggest a novel mechanism may be involved in chronic inflammatory progression of atherosclerosis with hyperhomocysteinemia. (C) 2002 Elsevier Science BY All rights reserved.

语种: 英语
WOS记录号: WOS:000175316800026
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57119
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Third Hosp, Inst Vasc Med, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Q,Zeng, XK,Guo, JX,et al. Oxidant stress mechanism of homocysteine potentiating Con A-induced proliferation in murine splenic T lymphocytes[J]. CARDIOVASCULAR RESEARCH,2002,53(4):1035-1042.
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