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学科主题: 公共卫生
题名:
Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice
作者: Zhang, Zhaofeng; Wang, Junbo; Dai, Xiaoqian; Ding, Ye; Li, Yong
关键词: craniofacial abnormality ; retinoic acid ; vitamin B12 ; whole embryo culture ; mouse
刊名: CLEFT PALATE-CRANIOFACIAL JOURNAL
发表日期: 2011-07-01
DOI: 10.1597/09-156
卷: 48, 期:4, 页:355-362
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Surgery
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Surgery
关键词[WOS]: NEURAL CREST CELLS ; BRANCHIAL ARCH MORPHOGENESIS ; MOUSE EMBRYO ; FOLIC-ACID ; CHICK-EMBRYO ; MEPM CELLS ; HINDBRAIN ; ENDOTHELIN-1 ; MIGRATION ; SEGMENTATION
英文摘要:

Objective: The purpose of the present study was to identify the potential effect of prenatal vitamin B12 administration on retinoic acid (RA)-induced early craniofacial abnormalities in mice and to investigate the possible mechanisms by which vitamin B12 reduces malformations.

Design: In our study, whole embryo culture was used to explore the effect of vitamin B12 on mouse embryos during the critical period of organogenesis. All embryos were exposed to 0.4 mu M RA and different concentrations of vitamin B12 and scored for their growth in the branchial region at the end of a 48-hour culture period. The endothelin-1 (ET-1)/dHAND protein expression levels in the first branchial arch were investigated using an immunohistochemical method.

Results: In the whole embryo culture, 100 and 10 mM vitamin B12 dose-dependently prevented branchial region malformations and decreased craniofacial defects by 90.5% and 77.3%, respectively. ET-1 and dHAND protein levels were significantly increased in vitamin B12-supplemented embryos compared to the RA-exposed group in embryonic branchial region.

Conclusions: These results suggest that vitamin B12 may prevent RA-induced craniofacial abnormalities via prevention of an RA-induced decrease of ET-1 and dHAND protein levels in the branchial region during the organogenic period. This study may shed new light on preventing craniofacial abnormalities.

语种: 英语
所属项目编号: 2006BAD27B01
项目资助者: Ministry of Science and Technology of the People&prime ; s Republic of China
WOS记录号: WOS:000296019900001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57120
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Zhaofeng,Wang, Junbo,Dai, Xiaoqian,et al. Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice[J]. CLEFT PALATE-CRANIOFACIAL JOURNAL,2011,48(4):355-362.
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