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Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice
Zhang, Zhaofeng; Wang, Junbo; Dai, Xiaoqian; Ding, Ye; Li, Yong
关键词Craniofacial Abnormality Retinoic Acid Vitamin B12 Whole Embryo Culture Mouse
刊名CLEFT PALATE-CRANIOFACIAL JOURNAL
2011-07-01
DOI10.1597/09-156
48期:4页:355-362
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Surgery
资助者Ministry of Science and Technology of the People&prime ; s Republic of China ; Ministry of Science and Technology of the People&prime ; s Republic of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Surgery
关键词[WOS]NEURAL CREST CELLS ; BRANCHIAL ARCH MORPHOGENESIS ; MOUSE EMBRYO ; FOLIC-ACID ; CHICK-EMBRYO ; MEPM CELLS ; HINDBRAIN ; ENDOTHELIN-1 ; MIGRATION ; SEGMENTATION
英文摘要

Objective: The purpose of the present study was to identify the potential effect of prenatal vitamin B12 administration on retinoic acid (RA)-induced early craniofacial abnormalities in mice and to investigate the possible mechanisms by which vitamin B12 reduces malformations.

Design: In our study, whole embryo culture was used to explore the effect of vitamin B12 on mouse embryos during the critical period of organogenesis. All embryos were exposed to 0.4 mu M RA and different concentrations of vitamin B12 and scored for their growth in the branchial region at the end of a 48-hour culture period. The endothelin-1 (ET-1)/dHAND protein expression levels in the first branchial arch were investigated using an immunohistochemical method.

Results: In the whole embryo culture, 100 and 10 mM vitamin B12 dose-dependently prevented branchial region malformations and decreased craniofacial defects by 90.5% and 77.3%, respectively. ET-1 and dHAND protein levels were significantly increased in vitamin B12-supplemented embryos compared to the RA-exposed group in embryonic branchial region.

Conclusions: These results suggest that vitamin B12 may prevent RA-induced craniofacial abnormalities via prevention of an RA-induced decrease of ET-1 and dHAND protein levels in the branchial region during the organogenic period. This study may shed new light on preventing craniofacial abnormalities.

语种英语
所属项目编号2006BAD27B01
资助者Ministry of Science and Technology of the People&prime ; s Republic of China ; Ministry of Science and Technology of the People&prime ; s Republic of China
WOS记录号WOS:000296019900001
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57120
Collection北京大学公共卫生学院
作者单位Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhaofeng,Wang, Junbo,Dai, Xiaoqian,et al. Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice[J]. CLEFT PALATE-CRANIOFACIAL JOURNAL,2011,48(4):355-362.
APA Zhang, Zhaofeng,Wang, Junbo,Dai, Xiaoqian,Ding, Ye,&Li, Yong.(2011).Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice.CLEFT PALATE-CRANIOFACIAL JOURNAL,48(4),355-362.
MLA Zhang, Zhaofeng,et al."Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice".CLEFT PALATE-CRANIOFACIAL JOURNAL 48.4(2011):355-362.
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