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Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice
Zhang, Zhaofeng; Wang, Junbo; Dai, Xiaoqian; Ding, Ye; Li, Yong
关键词Craniofacial Abnormality Retinoic Acid Vitamin B12 Whole Embryo Culture Mouse
刊名CLEFT PALATE-CRANIOFACIAL JOURNAL
2011-07-01
DOI10.1597/09-156
48期:4页:355-362
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Surgery
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Surgery
关键词[WOS]NEURAL CREST CELLS ; BRANCHIAL ARCH MORPHOGENESIS ; MOUSE EMBRYO ; FOLIC-ACID ; CHICK-EMBRYO ; MEPM CELLS ; HINDBRAIN ; ENDOTHELIN-1 ; MIGRATION ; SEGMENTATION
英文摘要

Objective: The purpose of the present study was to identify the potential effect of prenatal vitamin B12 administration on retinoic acid (RA)-induced early craniofacial abnormalities in mice and to investigate the possible mechanisms by which vitamin B12 reduces malformations.

Design: In our study, whole embryo culture was used to explore the effect of vitamin B12 on mouse embryos during the critical period of organogenesis. All embryos were exposed to 0.4 mu M RA and different concentrations of vitamin B12 and scored for their growth in the branchial region at the end of a 48-hour culture period. The endothelin-1 (ET-1)/dHAND protein expression levels in the first branchial arch were investigated using an immunohistochemical method.

Results: In the whole embryo culture, 100 and 10 mM vitamin B12 dose-dependently prevented branchial region malformations and decreased craniofacial defects by 90.5% and 77.3%, respectively. ET-1 and dHAND protein levels were significantly increased in vitamin B12-supplemented embryos compared to the RA-exposed group in embryonic branchial region.

Conclusions: These results suggest that vitamin B12 may prevent RA-induced craniofacial abnormalities via prevention of an RA-induced decrease of ET-1 and dHAND protein levels in the branchial region during the organogenic period. This study may shed new light on preventing craniofacial abnormalities.

语种英语
WOS记录号WOS:000296019900001
项目编号2006BAD27B01
资助机构Ministry of Science and Technology of the People&prime ; s Republic of China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57120
专题北京大学公共卫生学院
北京大学公共卫生学院_营养与食品卫生学系
北京大学公共卫生学院_公共卫生学院
作者单位Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Zhaofeng,Wang, Junbo,Dai, Xiaoqian,et al. Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice[J]. CLEFT PALATE-CRANIOFACIAL JOURNAL,2011,48(4):355-362.
APA Zhang, Zhaofeng,Wang, Junbo,Dai, Xiaoqian,Ding, Ye,&Li, Yong.(2011).Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice.CLEFT PALATE-CRANIOFACIAL JOURNAL,48(4),355-362.
MLA Zhang, Zhaofeng,et al."Prevention of Retinoic Acid-Induced Early Craniofacial Abnormalities by Vitamin B12 in Mice".CLEFT PALATE-CRANIOFACIAL JOURNAL 48.4(2011):355-362.
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