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学科主题: 药学
题名:
Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry
作者: Zhao, Xin1; Yang, Dong-Hui1; Zhou, Qi-Le2; Xu, Feng1; Zhang, Lei1; Liang, Jing1; Liu, Guang-Xue1; Cai, Shao-Qing1; Yang, Xiu-Wei1
关键词: Danshen decoction ; HPLC-DAD-ESI-IT-TOF ; MS ; phenolic acid ; metabolites ; WZS-miniature pig
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2013-06-01
DOI: 10.1002/bmc.2851
卷: 27, 期:6, 页:720-735
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SALVIANOLIC-ACID-B ; LIQUID-CHROMATOGRAPHY ; PHENOLIC-ACIDS ; TANSHINONE IIA ; RAT PLASMA ; MILTIORRHIZA ; CHEMISTRY ; PHARMACOKINETICS ; CRYPTOTANSHINONE ; CONSTITUENTS
英文摘要:

Danshen (DS) is a widely used traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases. A simple, rapid and sensitive method was developed for identification of the in vivo metabolites in urine of WZS-miniature pigs after oral administration of DS decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry. This method has been successfully applied to simultaneous identification of 50 compounds (including 11 new ones) in pig urine. In addition, one new compound, (3-hydroxyphenyl) crylic acid glycine methyl ester (C1), along with eight known ones were first isolated by column chromatography and identified by spectroscopic means, including 1D/2DNMR and mass spectrometry, as reference substances. Ten phenolic compounds (protocatechuic aldehyde, protocatechuic acid, caffeic acid, danshensu, ferulic acid, isoferulic acid, rosmarinic acid and salvianolic acid A/B/D) were found to be the main absorbed original constituents of DS decoction, which underwent the metabolic reactions of glucuronidation, sulfation, methylation, hydrogenation and glycine conjugation in vivo. In conclusion, the developed method is applicable to the analysis and identification of constituents in biological matrices after administration of DS decoction. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2009ZX09502-006 ; 2009ZX09301-010
项目资助者: &prime ; Major New Medicine Project&prime ; in Mega-projects of Science Research of China ; National Science and Technology Major Project for Major New Drugs Innovation and Development of China
WOS记录号: WOS:000317613200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57140
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

Recommended Citation:
Zhao, Xin,Yang, Dong-Hui,Zhou, Qi-Le,et al. Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(6):720-735.
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