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Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry
Zhao, Xin1; Yang, Dong-Hui1; Zhou, Qi-Le2; Xu, Feng1; Zhang, Lei1; Liang, Jing1; Liu, Guang-Xue1; Cai, Shao-Qing1; Yang, Xiu-Wei1
关键词Danshen Decoction Hplc-dad-esi-it-tof Ms Phenolic Acid Metabolites Wzs-miniature Pig
刊名BIOMEDICAL CHROMATOGRAPHY
2013-06-01
DOI10.1002/bmc.2851
27期:6页:720-735
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SALVIANOLIC-ACID-B ; LIQUID-CHROMATOGRAPHY ; PHENOLIC-ACIDS ; TANSHINONE IIA ; RAT PLASMA ; MILTIORRHIZA ; CHEMISTRY ; PHARMACOKINETICS ; CRYPTOTANSHINONE ; CONSTITUENTS
英文摘要

Danshen (DS) is a widely used traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases. A simple, rapid and sensitive method was developed for identification of the in vivo metabolites in urine of WZS-miniature pigs after oral administration of DS decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry. This method has been successfully applied to simultaneous identification of 50 compounds (including 11 new ones) in pig urine. In addition, one new compound, (3-hydroxyphenyl) crylic acid glycine methyl ester (C1), along with eight known ones were first isolated by column chromatography and identified by spectroscopic means, including 1D/2DNMR and mass spectrometry, as reference substances. Ten phenolic compounds (protocatechuic aldehyde, protocatechuic acid, caffeic acid, danshensu, ferulic acid, isoferulic acid, rosmarinic acid and salvianolic acid A/B/D) were found to be the main absorbed original constituents of DS decoction, which underwent the metabolic reactions of glucuronidation, sulfation, methylation, hydrogenation and glycine conjugation in vivo. In conclusion, the developed method is applicable to the analysis and identification of constituents in biological matrices after administration of DS decoction. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000317613200009
项目编号2009ZX09502-006 ; 2009ZX09301-010
资助机构&prime ; Major New Medicine Project&prime ; in Mega-projects of Science Research of China ; National Science and Technology Major Project for Major New Drugs Innovation and Development of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57140
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Xin,Yang, Dong-Hui,Zhou, Qi-Le,et al. Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(6):720-735.
APA Zhao, Xin.,Yang, Dong-Hui.,Zhou, Qi-Le.,Xu, Feng.,Zhang, Lei.,...&Yang, Xiu-Wei.(2013).Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,27(6),720-735.
MLA Zhao, Xin,et al."Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 27.6(2013):720-735.
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