北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis
作者: Huang, Jing1,2,3,4; Liu, Gang1,2,3,4; Zhang, Yi-miao1,2,3,4; Cui, Zhao1,2,3,4; Wang, Fang1,2,3,4; Liu, Xiao-Jing1,2,3,4; Chu, Rong1,2,3,4; Zhao, Ming-hui1,2,3,4,5
关键词: Focal segmental glomerulosclerosis ; Urinary soluble urokinase receptor ; Podocyte
刊名: BMC MEDICINE
发表日期: 2014-05-20
DOI: 10.1186/1741-7015-12-81
卷: 12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: PLASMINOGEN-ACTIVATOR RECEPTOR ; CELL-ADHESION ; GLOMERULAR-PERMEABILITY ; REDUCES PROTEINURIA ; PRIMARY FSGS ; PLASMA ; SUPAR ; VITRONECTIN ; EXPRESSION ; VARIANTS
英文摘要:

Background: Focal segmental glomerulosclerosis (FSGS) is a major cause of end-stage renal disease. Recent studies have proposed that plasma soluble urokinase receptor (suPAR) might be a causative circulating factor but this proposal has caused controversy. This study aimed to measure urinary suPAR levels in patients with primary FSGS and its significance in the pathogenesis of FSGS.

Methods: Sixty-two patients with primary FSGS, diagnosed between January 2006 and January 2012, with complete clinical and pathologic data were enrolled, together with disease and normal controls. Urinary suPAR levels were measured using commercial ELISA kits and were corrected by urinary creatinine (Cr). The associations between urinary suPAR levels and clinical data at presentation and during follow up were analyzed. Conditionally immortalized human podocytes were used to study the effect of urinary suPAR on activating beta 3 integrin detected by AP5 staining.

Results: The urinary suPAR level of patients with primary FSGS (500.56, IQR 262.78 to 1,059.44 pg/mu mol Cr) was significantly higher than that of patients with minimal change disease (307.86, IQR 216.54 to 480.18 pg/mu mol Cr, P = 0.033), membranous nephropathy (250.23, IQR 170.37 to 357.59 pg/mu mol Cr, P < 0.001), secondary FSGS (220.45, IQR 149.38 to 335.54 pg/mu mol Cr, P < 0.001) and normal subjects (183.59, IQR 103.92 to 228.78 pg/mu mol Cr, P < 0.001). The urinary suPAR level of patients with cellular variant was significantly higher than that of patients with tip variant. The urinary suPAR level in the patients with primary FSGS was positively correlated with 24-hour urine protein (r = 0.287, P = 0.024). During follow up, the urinary suPAR level of patients with complete remission decreased significantly (661.19, IQR 224.32 to 1,115.29 pg/mu mol Cr versus 217.68, IQR 121.77 to 415.55 pg/mu mol Cr, P = 0.017). The AP5 signal was strongly induced along the cell membrane when human differentiated podocytes were incubated with the urine of patients with FSGS at presentation, and the signal could be reduced by a blocking antibody specific to uPAR.

Conclusions: Urinary suPAR was specifically elevated in patients with primary FSGS and was associated with disease severity. The elevated urinary suPAR could activate beta 3 integrin on human podocytes.

语种: 英语
所属项目编号: 2012CB517702 ; 81321064
项目资助者: Chinese 973 project ; National Natural Science Foundation of China
WOS记录号: WOS:000338244600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57150
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Renal Div, Beijing 100871, Peoples R China
2.Peking Univ, Inst Nephrol, Beijing 100871, Peoples R China
3.Minist Hlth China, Key Lab Renal Dis, Beijing, Peoples R China
4.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
5.Minist Educ China, Key Lab CKD Prevent & Treatment, Beijing, Peoples R China

Recommended Citation:
Huang, Jing,Liu, Gang,Zhang, Yi-miao,et al. Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis[J]. BMC MEDICINE,2014,12.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Jing]'s Articles
[Liu, Gang]'s Articles
[Zhang, Yi-miao]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Jing]‘s Articles
[Liu, Gang]‘s Articles
[Zhang, Yi-miao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace