IR@PKUHSC  > 北京大学基础医学院  > 神经生物学系
学科主题基础医学
Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats
Wang, Ya-Nan1,2,3; Wang, Bo1,2,3; Liang, Ming3; Han, Cai-Yan3; Zhang, Bin3; Cai, Jie1,2; Sun, Wei3; Xing, Guo-Gang1,2,4,5
关键词Asthenozoospermia male infertility CatSper1 channel cyclophosphamide Sheng-Jing-San recipe
刊名FERTILITY AND STERILITY
2013-02-01
DOI10.1016/j.fertnstert.2012.10.030
99期:2页:579-587
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]MALE-FERTILITY ; SEMINAL PLASMA ; HYPERACTIVATED MOTILITY ; POSTNATAL-DEVELOPMENT ; GENE-EXPRESSION ; MOUSE TESTIS ; NULL SPERM ; CA2+ ENTRY ; CYCLOPHOSPHAMIDE ; INFERTILITY
英文摘要

Objective: To determine the expression of CatSper1 channel in epididymal spermatozoa in a rat model of asthenozoospermia, induced by cyclophosphamide (CP), and further examine the effects of soluble granules of Sheng-Jing-San (SJS), a traditional Chinese medicine recipe, on CatSper1 expression and sperm motility in the CP-induced asthenozoospermic rats.

Design: Placebo-controlled, randomized trial.

Setting: Neuroscience Research Institute, Peking University, China.

Animal(s): Sexually mature male Sprague-Dawley rats (n = 60).

Intervention(s): In the CP group, CP at the dose of 35 mg/kg intraperitoneally injected into rats once a day for 7 days; in the normal saline (NS) group, 0.9% saline solution was injected as control.

Main Outcome Measure(s): Sperm motility and count were evaluated by computer-assisted sperm assay (CASA); protein and mRNA expression of CatSper1 channel in epididymal spermatozoa was determined by Western blotting and quantitative real-time RT-PCR, respectively.

Result(s): The rats were randomly divided into five groups with 12 rats in each group: CP, normal saline (NS), CP + SJS, CP + NS, and treatment naive. In the CP + SJS group, after the last injection of CP, SJS at a dose of 30 mg/kg was intragastrically administrated to rats once a day for 14 days; in CP + NS group, saline solution instead of SJS was administrated as control. In the treatment naive group, rats were normally fed for 21 days as controls. We found a statistically significant reduction of the CatSper1 channel, which is associated with an impairment of sperm motility in the epididymal spermatozoa of CP-induced asthenozoospermic rats. Soluble granules of SJS could dramatically restore the CP-induced down-regulation of CatSper1 in epididymal spermatozoa, which greatly improved the sperm motility in the asthenozoospermic rats.

Conclusion(s): Down-regulation of the CatSper1 channel in epididymal spermatozoa likely contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by SJS improves sperm motility and thus can be used as an effective therapeutic strategy for the treatment of male infertility diagnosed with asthenozoospermia. (Fertil Steril (R) 2013; 99: 579-87. (C) 2013 by American Society for Reproductive Medicine.)

语种英语
WOS记录号WOS:000314662400049
项目编号31171063 ; 81072951 ; 7112079
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57164
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
北京大学第三临床医学院_呼吸科
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Neurosci, Beijing, Peoples R China
4.Minist Publ Hlth, Beijing, Peoples R China
5.Shandong Univ Chinese Med, Affiliated Hosp 2, Reprod Med Ctr, Jinan, Peoples R China
推荐引用方式
GB/T 7714
Wang, Ya-Nan,Wang, Bo,Liang, Ming,et al. Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats[J]. FERTILITY AND STERILITY,2013,99(2):579-587.
APA Wang, Ya-Nan.,Wang, Bo.,Liang, Ming.,Han, Cai-Yan.,Zhang, Bin.,...&Xing, Guo-Gang.(2013).Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats.FERTILITY AND STERILITY,99(2),579-587.
MLA Wang, Ya-Nan,et al."Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats".FERTILITY AND STERILITY 99.2(2013):579-587.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Down-regulation of C(593KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Ya-Nan]的文章
[Wang, Bo]的文章
[Liang, Ming]的文章
百度学术
百度学术中相似的文章
[Wang, Ya-Nan]的文章
[Wang, Bo]的文章
[Liang, Ming]的文章
必应学术
必应学术中相似的文章
[Wang, Ya-Nan]的文章
[Wang, Bo]的文章
[Liang, Ming]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。