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学科主题: 基础医学
题名:
Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats
作者: Wang, Ya-Nan1,2,3; Wang, Bo1,2,3; Liang, Ming3; Han, Cai-Yan3; Zhang, Bin3; Cai, Jie1,2; Sun, Wei3; Xing, Guo-Gang1,2,4,5
关键词: Asthenozoospermia ; male infertility ; CatSper1 channel ; cyclophosphamide ; Sheng-Jing-San recipe
刊名: FERTILITY AND STERILITY
发表日期: 2013-02-01
DOI: 10.1016/j.fertnstert.2012.10.030
卷: 99, 期:2, 页:579-587
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: MALE-FERTILITY ; SEMINAL PLASMA ; HYPERACTIVATED MOTILITY ; POSTNATAL-DEVELOPMENT ; GENE-EXPRESSION ; MOUSE TESTIS ; NULL SPERM ; CA2+ ENTRY ; CYCLOPHOSPHAMIDE ; INFERTILITY
英文摘要:

Objective: To determine the expression of CatSper1 channel in epididymal spermatozoa in a rat model of asthenozoospermia, induced by cyclophosphamide (CP), and further examine the effects of soluble granules of Sheng-Jing-San (SJS), a traditional Chinese medicine recipe, on CatSper1 expression and sperm motility in the CP-induced asthenozoospermic rats.

Design: Placebo-controlled, randomized trial.

Setting: Neuroscience Research Institute, Peking University, China.

Animal(s): Sexually mature male Sprague-Dawley rats (n = 60).

Intervention(s): In the CP group, CP at the dose of 35 mg/kg intraperitoneally injected into rats once a day for 7 days; in the normal saline (NS) group, 0.9% saline solution was injected as control.

Main Outcome Measure(s): Sperm motility and count were evaluated by computer-assisted sperm assay (CASA); protein and mRNA expression of CatSper1 channel in epididymal spermatozoa was determined by Western blotting and quantitative real-time RT-PCR, respectively.

Result(s): The rats were randomly divided into five groups with 12 rats in each group: CP, normal saline (NS), CP + SJS, CP + NS, and treatment naive. In the CP + SJS group, after the last injection of CP, SJS at a dose of 30 mg/kg was intragastrically administrated to rats once a day for 14 days; in CP + NS group, saline solution instead of SJS was administrated as control. In the treatment naive group, rats were normally fed for 21 days as controls. We found a statistically significant reduction of the CatSper1 channel, which is associated with an impairment of sperm motility in the epididymal spermatozoa of CP-induced asthenozoospermic rats. Soluble granules of SJS could dramatically restore the CP-induced down-regulation of CatSper1 in epididymal spermatozoa, which greatly improved the sperm motility in the asthenozoospermic rats.

Conclusion(s): Down-regulation of the CatSper1 channel in epididymal spermatozoa likely contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by SJS improves sperm motility and thus can be used as an effective therapeutic strategy for the treatment of male infertility diagnosed with asthenozoospermia. (Fertil Steril (R) 2013; 99: 579-87. (C) 2013 by American Society for Reproductive Medicine.)

语种: 英语
所属项目编号: 31171063 ; 81072951 ; 7112079
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000314662400049
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57164
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Neurosci, Beijing, Peoples R China
4.Minist Publ Hlth, Beijing, Peoples R China
5.Shandong Univ Chinese Med, Affiliated Hosp 2, Reprod Med Ctr, Jinan, Peoples R China

Recommended Citation:
Wang, Ya-Nan,Wang, Bo,Liang, Ming,et al. Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats[J]. FERTILITY AND STERILITY,2013,99(2):579-587.
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