北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma
作者: Ahmed, Sarah A.1,2,3; van de Sande, Wendy W. J.4; Desnos-Ollivier, Marie5; Fahal, Ahmed H.6; Mhmoud, Najwa A.6; de Hoog, G. S.7,8,9,10,11
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2015-10-01
DOI: 10.1128/JCM.01544-15
卷: 53, 期:10, 页:3280-3285
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: RECOMBINASE POLYMERASE AMPLIFICATION ; BLACK-GRAIN EUMYCETOMA ; RAPID IDENTIFICATION ; CAUSATIVE AGENTS ; DNA ; DIAGNOSIS ; BURDEN ; FUNGI ; LAMP
英文摘要:

Appropriate diagnosis and treatment of eumycetoma may vary significantly depending on the causative agent. To date, the most common fungus causing mycetoma worldwide is Madurella mycetomatis. This species fails to express any recognizable morphological characteristics, and reliable identification can therefore only be achieved with the application of molecular techniques. Recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) are proposed as alternatives to phenotypic methods. Species-specific primers were developed to target the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region of M. mycetomatis. Both isothermal amplification techniques showed high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques was assessed in comparison to conventional PCR using biopsy specimens from eumycetoma patients. RPA is reliable and easy to operate and has the potential to be implemented in areas where mycetoma is endemic. The techniques may be expanded to detect fungal DNA from environmental samples.

语种: 英语
WOS记录号: WOS:000365625300024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57174
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Khartoum, Fac Med Lab Sci, Khartoum, Sudan
2.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
3.Univ Khartoum, Mycetoma Res Ctr, Khartoum, Sudan
4.King Abdulaziz Univ, Jeddah 21413, Saudi Arabia
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands
6.Erasmus MC, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands
7.Inst Pasteur, Unite Mycol Mol, Ctr Natl Reference Mycol & Antifong, Paris, France
8.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
9.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
10.Second Mil Med Univ, Shanghai Inst Med Mycol, Changzheng Hosp, Shanghai, Peoples R China
11.Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil

Recommended Citation:
Ahmed, Sarah A.,van de Sande, Wendy W. J.,Desnos-Ollivier, Marie,et al. Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2015,53(10):3280-3285.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ahmed, Sarah A.]'s Articles
[van de Sande, Wendy W. J.]'s Articles
[Desnos-Ollivier, Marie]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ahmed, Sarah A.]‘s Articles
[van de Sande, Wendy W. J.]‘s Articles
[Desnos-Ollivier, Marie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace