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学科主题临床医学
Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma
Ahmed, Sarah A.1,2,3; van de Sande, Wendy W. J.4; Desnos-Ollivier, Marie5; Fahal, Ahmed H.6; Mhmoud, Najwa A.6; de Hoog, G. S.7,8,9,10,11
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2015-10-01
DOI10.1128/JCM.01544-15
53期:10页:3280-3285
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]RECOMBINASE POLYMERASE AMPLIFICATION ; BLACK-GRAIN EUMYCETOMA ; RAPID IDENTIFICATION ; CAUSATIVE AGENTS ; DNA ; DIAGNOSIS ; BURDEN ; FUNGI ; LAMP
英文摘要

Appropriate diagnosis and treatment of eumycetoma may vary significantly depending on the causative agent. To date, the most common fungus causing mycetoma worldwide is Madurella mycetomatis. This species fails to express any recognizable morphological characteristics, and reliable identification can therefore only be achieved with the application of molecular techniques. Recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) are proposed as alternatives to phenotypic methods. Species-specific primers were developed to target the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region of M. mycetomatis. Both isothermal amplification techniques showed high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques was assessed in comparison to conventional PCR using biopsy specimens from eumycetoma patients. RPA is reliable and easy to operate and has the potential to be implemented in areas where mycetoma is endemic. The techniques may be expanded to detect fungal DNA from environmental samples.

语种英语
WOS记录号WOS:000365625300024
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57174
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Univ Khartoum, Fac Med Lab Sci, Khartoum, Sudan
2.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
3.Univ Khartoum, Mycetoma Res Ctr, Khartoum, Sudan
4.King Abdulaziz Univ, Jeddah 21413, Saudi Arabia
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands
6.Erasmus MC, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands
7.Inst Pasteur, Unite Mycol Mol, Ctr Natl Reference Mycol & Antifong, Paris, France
8.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
9.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
10.Second Mil Med Univ, Shanghai Inst Med Mycol, Changzheng Hosp, Shanghai, Peoples R China
11.Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil
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GB/T 7714
Ahmed, Sarah A.,van de Sande, Wendy W. J.,Desnos-Ollivier, Marie,et al. Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2015,53(10):3280-3285.
APA Ahmed, Sarah A.,van de Sande, Wendy W. J.,Desnos-Ollivier, Marie,Fahal, Ahmed H.,Mhmoud, Najwa A.,&de Hoog, G. S..(2015).Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma.JOURNAL OF CLINICAL MICROBIOLOGY,53(10),3280-3285.
MLA Ahmed, Sarah A.,et al."Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma".JOURNAL OF CLINICAL MICROBIOLOGY 53.10(2015):3280-3285.
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