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学科主题: 基础医学
题名:
Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells
作者: Huang, Yazi; Zhao, Beilei; Liu, Yahan; Wang, Nanping
刊名: PPAR RESEARCH
发表日期: 2014
DOI: 10.1155/2014/740121
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: INFLAMMATORY ADHESION MOLECULES ; LYSOPHOSPHATIDIC ACID ; PPAR-GAMMA ; CARDIOVASCULAR-SYSTEM ; TISSUE DISTRIBUTION ; LIPOPROTEIN ; OVEREXPRESSION ; IDENTIFICATION ; BIOLOGY ; LIGAND
英文摘要:

Lipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 remains largely unknown. Here, we aimed to examine a role of peroxisome proliferator-activated receptor gamma (PPAR gamma.)in the transcriptional control of LPP1 gene expression. In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPAR gamma increased the mRNA level of LPP1. Chromatin immunoprecipitation assay showed that PPAR.. binds to the putative PPAR-responsive elements (PPREs) within the 5′-flanking region of the human LPP1 gene. Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPAR.. and rosiglitazone, a specific ligand for PPAR.., could significantly upregulate the reporter activity. However, site-directed mutagenesis of the PPRE motif abolished the induction. In conclusion, our results demonstrated that PPAR.. transcriptionally activated the expression of LPP1 gene in ECs, suggesting a potential role of PPAR.. in the metabolism of phospholipids.

语种: 英语
所属项目编号: 81220108005 ; 81221061 ; 2010CB912500
项目资助者: National Science Foundation of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000336288700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57185
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Yazi,Zhao, Beilei,Liu, Yahan,et al. Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells[J]. PPAR RESEARCH,2014.
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