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学科主题基础医学
Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells
Huang, Yazi; Zhao, Beilei; Liu, Yahan; Wang, Nanping
刊名PPAR RESEARCH
2014
DOI10.1155/2014/740121
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]INFLAMMATORY ADHESION MOLECULES ; LYSOPHOSPHATIDIC ACID ; PPAR-GAMMA ; CARDIOVASCULAR-SYSTEM ; TISSUE DISTRIBUTION ; LIPOPROTEIN ; OVEREXPRESSION ; IDENTIFICATION ; BIOLOGY ; LIGAND
英文摘要

Lipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 remains largely unknown. Here, we aimed to examine a role of peroxisome proliferator-activated receptor gamma (PPAR gamma.)in the transcriptional control of LPP1 gene expression. In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPAR gamma increased the mRNA level of LPP1. Chromatin immunoprecipitation assay showed that PPAR.. binds to the putative PPAR-responsive elements (PPREs) within the 5′-flanking region of the human LPP1 gene. Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPAR.. and rosiglitazone, a specific ligand for PPAR.., could significantly upregulate the reporter activity. However, site-directed mutagenesis of the PPRE motif abolished the induction. In conclusion, our results demonstrated that PPAR.. transcriptionally activated the expression of LPP1 gene in ECs, suggesting a potential role of PPAR.. in the metabolism of phospholipids.

语种英语
WOS记录号WOS:000336288700001
项目编号81220108005 ; 81221061 ; 2010CB912500
资助机构National Science Foundation of China ; Ministry of Science and Technology of China
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57185
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
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Huang, Yazi,Zhao, Beilei,Liu, Yahan,et al. Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells[J]. PPAR RESEARCH,2014.
APA Huang, Yazi,Zhao, Beilei,Liu, Yahan,&Wang, Nanping.(2014).Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells.PPAR RESEARCH.
MLA Huang, Yazi,et al."Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells".PPAR RESEARCH (2014).
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