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学科主题: 基础医学
题名:
Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape
作者: Xu, Xiaofeng1,2; Zeng, Zhu2,3,4,5; Yao, Weijuan2; Wang, Xianwei2; Sun, Dagong6; Ka, Weibo6; Zhang, Yingyu2; Wang, Xifu7; Chen, Xiaopeng8; Zha, Yi9; Sun, Li9; Xieg, Lide8; Wen, Zongyao2; Chien, Shu5
关键词: Dendritic cells ; Biomechanics ; Immunology ; Proteomics ; K562
刊名: JOURNAL OF BIOMECHANICS
发表日期: 2010-08-26
DOI: 10.1016/j.jbiomech.2010.04.028
卷: 43, 期:12, 页:2339-2347
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Engineering, Biomedical
研究领域[WOS]: Biophysics ; Engineering
关键词[WOS]: MURINE ERYTHROLEUKEMIA-CELLS ; MYELOID-LEUKEMIA ; T-CELLS ; MIGRATION ; METALLOPROTEINASES ; REORGANIZATION ; CYTOSKELETON ; MATURATION ; STIMULATE ; PROFILIN
英文摘要:

Dendritic cells (DCs), which are potent antigen presenting cells (APCs), are utilized to deliver the signals essential for the initiation of immune responses. In this study, we used an interdisciplinary approach to characterize the effect of K562 cells, a human chronic myeloid leukemia (CML) cell line, on the biomechanical characteristics and immune functions of DCs. When co-cultured with K562 cells, the biomechanical and immunological characteristics of immature DCs (imDCs) and mature DCs (mDCs) were severely impaired compared with controls. The changes include increased membrane viscoelasticity, reorganized cytoskeleton (F-actin), suppressed capability of antigen uptake, transen-dothelium migration, and activation of naive T cells. In exploring the mechanisms of these changes, we identified several genes and proteins by microarray analysis and 2D gel electrophoresis. Changes were found in the cytoskeleton-related genes and proteins (such as cofilin1 and profilin1) and matrix-related genes and proteins (such as TIMP1 and MMP9). These findings provide a molecular basis for the biomechanical and immunological changes of DCs in response to K562 and may help to elucidate the mechanism for tumor immune escape. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 10572007 ; 30770532
项目资助者: National Natural Science Foundations of China
WOS记录号: WOS:000282112300015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57191
Appears in Collections:基础医学院_北京大学血液流变中心_期刊论文

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作者单位: 1.Guiyang Med Coll, Guiyang 550004, Guizhou, Peoples R China
2.Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Hemorheol Ctr, Beijing 100191, Peoples R China
4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
5.Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
6.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Beijing 100191, Peoples R China
7.Peking Univ, Shougang Hosp, Dept Cardiol, Beijing 100144, Peoples R China
8.Chengde Med Coll, Chengde 067000, Peoples R China
9.Beijing Red Cross Blood Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Xu, Xiaofeng,Zeng, Zhu,Yao, Weijuan,et al. Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape[J]. JOURNAL OF BIOMECHANICS,2010,43(12):2339-2347.
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