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学科主题: 临床医学
题名:
THE GLUTAMINERGIC, GABAergic, DOPAMINERGIC BUT NOT CHOLINERGIC NEURONS ARE SUSCEPTIBLE TO ANAESTHESIA-INDUCED CELL DEATH IN THE RAT DEVELOPING BRAIN
作者: Zhou, Z. -W.1,2; Shu, Y.1; Li, M.2; Guo, X.2; Pac-Soo, C.1; Maze, M.3; Ma, D.1
关键词: anaesthetics ; neurons ; apoptosis ; nitrous oxide ; isoflurane ; neuronal development
刊名: NEUROSCIENCE
发表日期: 2011-02-03
DOI: 10.1016/j.neuroscience.2010.10.009
卷: 174, 页:64-70
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM ; GENERAL-ANESTHETICS ; BASAL GANGLIA ; SEROTONIN/DOPAMINE INTERACTION ; NEUROCOGNITIVE FUNCTION ; ALZHEIMERS-DISEASE ; REMOTE MEMORIES ; EARLY EXPOSURE ; ISOFLURANE ; ACETYLCHOLINE
英文摘要:

Neuronal cell death induced by anaesthetics in the developing brain was evident in previous pre-clinical studies. However, the neuronal cell types involved in anaesthesia-induced neuronal cell death remains elusive. The aim of this study was to investigate glutamatergic, GABAergic, cholinergic and dopaminergic neuronal cell apoptosis induced by anaesthetic exposure in specific brain regions in rats. Separate cohorts of 7-day-old Sprague Dawley (SD) rat pups were randomly assigned to two groups: Naive and anaesthetics alone (70% nitrous oxide and 0.75% isoflurane exposure for 6 h). The brains were sectioned and the slices that contained the basal forebrain, substantia nigra, cornu ammonis area 1 (CA1) subarea of hippocampus or cingulate cortex were selected and subsequently subjected to double-labelled fluorescent immunohistochemistry for choline acetyltransferase, dopamine, vesicular glutamate transporter 1 (vGLUT1) or glutamic acid decarboxylase 67 (GAD67) together with caspase 3, respectively. Compared to the naive control, anaesthetic exposure significantly increased the number of caspase-3 positive cells in the CA1 subarea of hippocampus, cingulate cortex, and substantia nigra, but not in the basal forebrain. 54% and 14% of apoptotic cells in the CA1 subarea of hippocampus were GABAergic and glutamatergic neurons respectively. In the cingulate cortex, 30% and 37% of apoptotic cells were GABAergic and glutamatergic neurons respectively. In the substantia nigra, 22% of apoptotic cells were dopaminergic neurons. Our data suggests, anaesthetic exposure significantly increases neuroapoptosis of glutamatergic, GABAergic and dopaminergic neurons in the developing brain but not that of the cholinergic neurons in the basal forebrain. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.

语种: 英语
项目资助者: Westminster Medical School Research Trust/Chelsea ; Westminster NHS Trust, London, UK
WOS记录号: WOS:000286558000006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57193
Appears in Collections:北京大学第三临床医学院_麻醉科_期刊论文

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作者单位: 1.Univ London Imperial Coll Sci Technol & Med, Chelsea & Westminster Hosp, Sect Anaesthet Pain Med & Intens Care, Dept Surg & Canc,Fac Med, London SW10 9NH, England
2.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100871, Peoples R China
3.UCSF, Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA

Recommended Citation:
Zhou, Z. -W.,Shu, Y.,Li, M.,et al. THE GLUTAMINERGIC, GABAergic, DOPAMINERGIC BUT NOT CHOLINERGIC NEURONS ARE SUSCEPTIBLE TO ANAESTHESIA-INDUCED CELL DEATH IN THE RAT DEVELOPING BRAIN[J]. NEUROSCIENCE,2011,174:64-70.
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