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学科主题: 临床医学
题名:
Mammalian Tolloid alters subcellular localization, internalization, and signaling of alpha(1a)-adrenergic receptors
作者: Xu, Qi; Xu, Ning; Zhang, Tan; Zhang, Hui; Li, Zijian; Yin, Feng; Lu, Zhizhen; Han, Qide; Zhang, Youyi
刊名: MOLECULAR PHARMACOLOGY
发表日期: 2006-08-01
DOI: 10.1124/mol.105.016451
卷: 70, 期:2, 页:532-541
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: BONE MORPHOGENETIC PROTEIN-1 ; PROCOLLAGEN C-PROTEINASE ; LAMININ-5 GAMMA-2 CHAIN ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; ALPHA(1B)-ADRENERGIC RECEPTOR ; CELLULAR-LOCALIZATION ; ADRENERGIC-RECEPTORS ; PC12 CELLS ; KINASE ; AGONIST
英文摘要:

In the present study, we identified the CUB5 domain of mammalian Tolloid (mTLD) as a novel protein binding to alpha(1A)-adrenergic receptor (AR) using the yeast two-hybrid system. Whereas CUB5 did not couple to either alpha(1B)-AR or alpha(1D)-AR. It was determined that amino acids 322 to 359 of alpha(1A)-AR were the major binding region for CUB5. The direct interaction between alpha(1A)-AR cytoplasmic tail and CUB5 was discovered by glutathione S-transferase pull-down assay. We confirmed the interaction of mTLD with alpha(1A)-AR in human embryonic kidney (HEK) 293 cells by immunoprecipitation, immunofluorescence, and fluorescence resonance energy transfer. Although mTLD did not affect the density and affinity of receptors in crudely prepared membranes from HEK293 cells stably expressing alpha(1A)-AR, it significantly altered the subcellular localization of the receptors. Moreover, mTLD reduced the level of cell surface alpha(1A)-ARs, delayed the initial rate of agonist-induced receptor internalization, and facilitated agonist-induced calcium transient. We have demonstrated that mTLD interacts with alpha(1A)-AR directly, alters the subcellular localization of receptor, and influences agonist-induced alpha(1A)-AR internalization and calcium signaling.

语种: 英语
WOS记录号: WOS:000239117900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57198
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China

Recommended Citation:
Xu, Qi,Xu, Ning,Zhang, Tan,et al. Mammalian Tolloid alters subcellular localization, internalization, and signaling of alpha(1a)-adrenergic receptors[J]. MOLECULAR PHARMACOLOGY,2006,70(2):532-541.
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