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学科主题: 临床医学
题名:
Interleukin-1 induces beta-calcitonin gene-related peptide secretion in human type II alveolar epithelial cells
作者: Li, WJ; Hou, LF; Hua, ZW; Wang, X
关键词: neuroimmunology ; neuropeptide ; inflammation ; chemokine
刊名: FASEB JOURNAL
发表日期: 2004-08-01
DOI: 10.1096/fj.04-1737fje
卷: 18, 期:11, 页:1603-+
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
关键词[WOS]: PROTEIN-KINASE-C ; NEUROEPITHELIAL BODIES ; AIRWAY HYPERRESPONSIVENESS ; RESPIRATORY-TRACT ; SENSORY NEURONS ; NEONATAL-RATS ; EXPRESSION ; CGRP ; LYMPHOCYTES ; RELEASE
英文摘要:

Calcitonin gene-related peptide ( CGRP) is a 37-amino acid neuropeptide mainly present in sensory nerve fibers, which is present in almost all organs, but it is also found in cultured rat type II alveolar epithelial cells (AEII). Our data have previously shown that CGRP may play an important role in inflammation as an immunomodulator. Proinflammatory factor IL-1 induces CGRP release from neuron-derived sources. However, whether IL-1 can induce CGRP secretion from a nonneural source, AEII cells, is not known. In the present study, we demonstrated that human AEII A549 cells expressed beta-CGRP, and IL-1beta (0.001- 50 ng/ml) directly increased CGRP secretion from these cells in a time- and concentration-dependent manner. The mRNA level of beta-CGRP was also elevated by IL-1beta ( 1 ng/ml). In addition, we found that IL-1beta-induced CGRP production was mediated through the PKC-p38 mitogen-activated protein (MAP) kinase-NF-kappaB signaling pathway. Furthermore, IL-1beta-induced chemokines MCP-1 and IL-8 were partially inhibited by exogenous hCGRP (0.1- 10 nM) and potentiated by hCGRP(8-37) ( 0.1- 10 nM), a CGRP(1)-receptor antagonist. In addition, the CGRP-inhibited chemokine effect was partially reduced by Rp-cAMP, a cAMP-PK inhibitor. These results suggest that AEII-derived CGRP may act in an autocrine/paracrine mode and play an important inhibitory role in the local area in lung inflammatory diseases.

语种: 英语
WOS记录号: WOS:000223554900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57203
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China

Recommended Citation:
Li, WJ,Hou, LF,Hua, ZW,et al. Interleukin-1 induces beta-calcitonin gene-related peptide secretion in human type II alveolar epithelial cells[J]. FASEB JOURNAL,2004,18(11):1603-+.
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