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学科主题: 临床医学
题名:
Protective effect of taurine on hypochlorous acid toxicity to nuclear nucleoside triphosphatase in isolated nuclei from rat liver
作者: Li, Ju-Xiang2; Pang, Yong-Zheng3; Tang, Chao-Shu3; Li, Zai-Quan1
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-03-01
卷: 10, 期:5, 页:694-698
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: Taurine has been shown to be an effective scavenger of hypochlorous acid (HOCl). The role of HOCl is well established in tissue damage associated with inflammation and injury. In the present study, the effect of HOCl on nuclear nucleoside triphosphatase of hepatocytes and the ability of taurine to prevent this effect were investigated.

METHODS: Isolated hepatic nuclei from rat liver were exposed to HOCl with or without taurine. The NTPase activity on nuclear envelope was assayed using ATP and GTP as substrates, respectively.

RESULTS: The first series of experiments evaluated the toxicity of HOCl and the efficacy of taurine to protect NTPase. HOCl at 10(-9)-5x10(-6) mol/L reduced nuclear NTPase activities in a concentration dependent manner (ATP and GTP as substrates) (P<0.01). HOCl at 10(-6) mol/L reduced the NTPase activity by 65% (ATP as substrate) and 76% (GTP as substrate). Taurine (10(-7) to 10(-4) mol/L) was tested for protection against HOCl at 10(-6) mol/L and the nuclei treated with 5x10(-4) mol/L taurine exhibited only 20% and 12% reduction in NTPase activities compared to untreated controls. A second study was performed comparing taurine to glutathione (GSH). GSH and HOCl at 10(-6) mol/L exhibited 46% and 67.4% reduction in NTPase activities compared with control. GSH (10(-4) mol/L) which was incubated with the nuclei and HOCl still exhibited 44.2% and 44.8% reduction in NTPase activities of untreated control. Taurine with HOCl only exhibited 15.2% and 17.1% reduction in NTPase activities, which provided more powerful protection against HOCl than GSH. The third experiment was undertaken to evaluate the specificity of taurine against HOCl. Incubation of rat hepatic nuclei with Fe(3+)/H(2)O(2) (1 m mol/L vs 5 mu mol/L) resulted in a decrease in nuclear NTPase activities (P<0.01). When hepatic nuclei were incubated with Tau (10(-4) mol/L) and Fe(3+)/H(2)O(2) (1m mol/L vs 5 mu mol/L), nuclear NTPase activities were only slightly increased as compared with that of incubation with Fe(3+)/H(2)O(2) alone. However, GSH failed to alter the NTPase activities induced by Fe(3+)/H(2)O(2).

CONCLUSION: The present findings indicate that HOCl can act as an inhibitor of nuclear NTPase. Taurine can antagonistically reduce the toxicity of HOCl to NTPase.

语种: 英语
所属项目编号: G2000056905 ; 30070308
项目资助者: Major State Basic Research Development Program of People&prime ; s Republic of China ; National Natural Science Foundation of China
WOS记录号: WOS:000208096600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57211
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China

Recommended Citation:
Li, Ju-Xiang,Pang, Yong-Zheng,Tang, Chao-Shu,et al. Protective effect of taurine on hypochlorous acid toxicity to nuclear nucleoside triphosphatase in isolated nuclei from rat liver[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(5):694-698.
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