北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Rapid Determination of Bile Acids in Bile from Various Mammals by Reversed-Phase Ultra-Fast Liquid Chromatography
作者: Si, Gu Leng Ri1,2; Yao, Peng1; Shi, Luwen1
刊名: JOURNAL OF CHROMATOGRAPHIC SCIENCE
发表日期: 2015-08-01
DOI: 10.1093/chromsci/bmu167
卷: 53, 期:7, 页:1060-1065
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: HUMAN PLASMA ; URSODEOXYCHOLIC ACID ; N-ACYLTRANSFERASE ; MASS-SPECTROMETRY ; BIOLOGICAL-FLUIDS ; NUCLEAR RECEPTOR ; SERUM ; LOCALIZATION ; CHOLESTEROL ; EXPRESSION
英文摘要:

A valid and efficient reversed-phase ultra-fast liquid chromatography method was developed for the simultaneous determination of 13 bile acids in the bile of three mammal species, including rat, pig and human gallstone patients. Chromatographic separation was performed with a Shim-pack XR-ODS column, and the mobile phase consisted of acetonitrile and potassium phosphate buffer (pH 2.6) at a flow rate of 0.5 mL min(-1). The linear detection range of most bile acids ranged from 2 to 600 ng mu L-1 with a good correlation coefficient (>0.9995). The precision of each bile acid was <1.8% for intraday and <4.8% for interday. All bile acids were separated in 15 min with satisfactory resolution, and the total analysis time was 18 min, including equilibration. The method was successfully applied in rapid screening of bile samples from the three mammals. Significant metabolic frameworks of bile acids among various species were observed, whereas considerable quantitative variations in both inter-and intraspecies were also observed, especially for gallstone patients. Our results suggest that detecting the change of bile acid profiles could be applied for the diagnosis of gallstone disease.

语种: 英语
项目资助者: Peking University ; Inner Mongolia People&prime ; s Hospital
WOS记录号: WOS:000363983400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57215
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Pharm Adm & Clin Pharm, Beijing 100191, Peoples R China
2.Inner Mongolia Autonomous Reg Peoples Hosp, Inner Mongolia Autonomou 010010, Hohhot, Peoples R China

Recommended Citation:
Si, Gu Leng Ri,Yao, Peng,Shi, Luwen. Rapid Determination of Bile Acids in Bile from Various Mammals by Reversed-Phase Ultra-Fast Liquid Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(7):1060-1065.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Si, Gu Leng Ri]'s Articles
[Yao, Peng]'s Articles
[Shi, Luwen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Si, Gu Leng Ri]‘s Articles
[Yao, Peng]‘s Articles
[Shi, Luwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace