学科主题基础医学
Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells
Li, Yin; Zhang, Yan; Furuyama, Kazumichi; Yokoyama, Satoru; Takeda, Kazuhisa; Shibahara, Shigeki; Takahashi, Kazuhiro
关键词adrenomedullin adipocytes differentiation gene expression
刊名PEPTIDES
2006-06-01
DOI10.1016/j.peptides.2005.11.009
27期:6页:1405-1414
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]NECROSIS-FACTOR-ALPHA ; SMOOTH-MUSCLE-CELLS ; ENDOGENOUS ADRENOMEDULLIN ; NATRIURETIC-PEPTIDE ; INSULIN-RESISTANCE ; TRANSCRIPTIONAL REGULATION ; AUTOCRINE/PARACRINE ROLE ; HYPOTENSIVE PEPTIDE ; VASOACTIVE PEPTIDE ; ADIPOSE CONVERSION
英文摘要

Adrenomedullin (AM), a potent vasodilator peptide, has been suggested to act against cardiovascular complications and insulin resistance in the metabolic syndrome. We have already reported the AM gene repression in the early phase of adipocyte differentiation of NIH 3T3-L1 cells. Here we show adipocyte differentiation-related regulatory element for AM gene repression (ADRE-AR) in 36-bp region (-2135/-2100) of the AM gene. 3T3-L1 cells were induced to differentiate to adipocytes by insulin, dexamethasone and 3-isobutyl-1-methylxanthine. On the third day of differentiation, the promoter function was analyzed using the reporter plasmids, which contain the promoter region of AM gene (-4616/+108) in pGL3-basic luciferase reporter vector. The promoter activity decreased to about 20% in 3T3-L1 adipocytes when compared with 3T3-L1 preadipocytes, and a 36-bp region (-2135 to -2100) upstream from the transcription initiation site of the AM gene was necessary for higher AM gene expression in preadipocytes. This 36-bp ADRE-AR contains three copies of G/AAAA sequence (5′-GAAATGAAAGTAAAA-3′) (-2124/-2110), which are conserved between mouse and human, and the introduction of mutations in each copy of G/AAAA sequence decreased the promoter activity in preadipocytes and adipocytes. Electrophoretic mobility shift assay showed that the full-length ADRE-AR was specifically bound by a certain nuclear protein(s). The present study has raised the possibility that ADRE-AR may play important roles in the AM gene expression in preadipocytes, and that the AM gene may be repressed through the ADRE-AR in adipocytes. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000238455600031
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57226
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Sendai, Miyagi 9808575, Japan
3.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Yin,Zhang, Yan,Furuyama, Kazumichi,et al. Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells[J]. PEPTIDES,2006,27(6):1405-1414.
APA Li, Yin.,Zhang, Yan.,Furuyama, Kazumichi.,Yokoyama, Satoru.,Takeda, Kazuhisa.,...&Takahashi, Kazuhiro.(2006).Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells.PEPTIDES,27(6),1405-1414.
MLA Li, Yin,et al."Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells".PEPTIDES 27.6(2006):1405-1414.
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