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学科主题: 基础医学
题名:
Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells
作者: Li, Yin; Zhang, Yan; Furuyama, Kazumichi; Yokoyama, Satoru; Takeda, Kazuhisa; Shibahara, Shigeki; Takahashi, Kazuhiro
关键词: adrenomedullin ; adipocytes ; differentiation ; gene ; expression
刊名: PEPTIDES
发表日期: 2006-06-01
DOI: 10.1016/j.peptides.2005.11.009
卷: 27, 期:6, 页:1405-1414
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: NECROSIS-FACTOR-ALPHA ; SMOOTH-MUSCLE-CELLS ; ENDOGENOUS ADRENOMEDULLIN ; NATRIURETIC-PEPTIDE ; INSULIN-RESISTANCE ; TRANSCRIPTIONAL REGULATION ; AUTOCRINE/PARACRINE ROLE ; HYPOTENSIVE PEPTIDE ; VASOACTIVE PEPTIDE ; ADIPOSE CONVERSION
英文摘要:

Adrenomedullin (AM), a potent vasodilator peptide, has been suggested to act against cardiovascular complications and insulin resistance in the metabolic syndrome. We have already reported the AM gene repression in the early phase of adipocyte differentiation of NIH 3T3-L1 cells. Here we show adipocyte differentiation-related regulatory element for AM gene repression (ADRE-AR) in 36-bp region (-2135/-2100) of the AM gene. 3T3-L1 cells were induced to differentiate to adipocytes by insulin, dexamethasone and 3-isobutyl-1-methylxanthine. On the third day of differentiation, the promoter function was analyzed using the reporter plasmids, which contain the promoter region of AM gene (-4616/+108) in pGL3-basic luciferase reporter vector. The promoter activity decreased to about 20% in 3T3-L1 adipocytes when compared with 3T3-L1 preadipocytes, and a 36-bp region (-2135 to -2100) upstream from the transcription initiation site of the AM gene was necessary for higher AM gene expression in preadipocytes. This 36-bp ADRE-AR contains three copies of G/AAAA sequence (5′-GAAATGAAAGTAAAA-3′) (-2124/-2110), which are conserved between mouse and human, and the introduction of mutations in each copy of G/AAAA sequence decreased the promoter activity in preadipocytes and adipocytes. Electrophoretic mobility shift assay showed that the full-length ADRE-AR was specifically bound by a certain nuclear protein(s). The present study has raised the possibility that ADRE-AR may play important roles in the AM gene expression in preadipocytes, and that the AM gene may be repressed through the ADRE-AR in adipocytes. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000238455600031
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57226
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Sendai, Miyagi 9808575, Japan
2.Tohoku Univ, Sch Hlth Sci, Dept Analyt Med Technol, Sendai, Miyagi 9808575, Japan
3.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Li, Yin,Zhang, Yan,Furuyama, Kazumichi,et al. Identification of adipocyte differentiation-related regulatory element for adrenomedullin gene repression (ADRE-AR) in 3T3-L1 cells[J]. PEPTIDES,2006,27(6):1405-1414.
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