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学科主题药学
Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry
Li, Yang1; Zhou, Guisheng1; Xing, Shihua1; Tu, Pengfei2; Li, Xiaobo1
关键词Echinacoside Human Intestinal Bacteria Uplc-q-tof-ms Metabolites Metabolic Pathway
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
2015-08-05
DOI10.1021/acs.jafc.5b02881
63期:30页:6764-6771
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]FECAL MICROBIAL-METABOLISM ; IN-VITRO ; ANTIOXIDANT ACTIVITY ; PHENYLETHANOID GLYCOSIDES ; CAFFEIC ACID ; BIOLOGICAL-ACTIVITIES ; PARKINSONS-DISEASE ; CISTANCHE-TUBULOSA ; PHENOLIC-COMPOUNDS ; ROOT EXTRACT
英文摘要

Echinacoside (ECH) is one of the representative phenylethanoid glycosides. It is widely present in plants and exhibits various bioactivities. However, the extremely low oral bioavailability of ECH in rats implies that ECH may go through multiple hydrolysis steps in the gastrointestinal tract prior to its absorption into the blood. Therefore, the gastrointestinal metabolites of ECH are more likely to be the bioactive components. This study established an approach combining ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with MSE technology and MetaboLynx software for rapid analysis of the ECH metabolic profile produced by human intestinal bacteria. As a result, 13 ECH metabolites and 5 possible metabolic pathways (including deglycosylation, dehydroxylation, reduction, hydroxylation, and acetylation) were identified. Furthermore, hydroxytyrosol (HT) and 3-hydroxyphenylpropionic acid (3-HPP) were found to be the two bioactive metabolites of ECH produced by human intestinal bacteria.

语种英语
WOS记录号WOS:000359279300015
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57238
Collection北京大学药学院
作者单位1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Yang,Zhou, Guisheng,Xing, Shihua,et al. Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(30):6764-6771.
APA Li, Yang,Zhou, Guisheng,Xing, Shihua,Tu, Pengfei,&Li, Xiaobo.(2015).Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,63(30),6764-6771.
MLA Li, Yang,et al."Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.30(2015):6764-6771.
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