|Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity|
|Yin Jing2; Yang Zheng3; Cao Yong-ping1; Ge Zi-gang2,4|
|关键词||Osteoarthritis Chondrocyte Differentiation|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||HUMAN ARTICULAR CHONDROCYTES ; GENE-EXPRESSION ; IN-VITRO ; II COLLAGEN ; PHENOTYPE ; CULTURE ; TISSUE ; VIVO ; QUANTIFICATION ; AGGRECANASE-1|
Background There is a difficulty in evaluating the in vivo functionality of individual chondrocytes, and there is much heterogeneity among cartilage affected by osteoarthritis (OA). In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis.
Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan (GAG) content were measured. Cell morphology, proliferation, and gene expression of collagen type I, II, and X, aggrecan, matrix metalloproteinase 13 (MMP-13), and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture.
Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable.
Conclusions Expression of functional genes such as collagen type II and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration. Chin Med J 2011;124(24):4245-4253
|项目编号||2011CB707500 ; 2012CB619102 ; 30772198 ; 30970881 ; 31011140348|
|资助机构||National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Hosp 1, Dept Orthopaed, Beijing 100034, Peoples R China|
2.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
3.Natl Univ Singapore, Yong Loo Lin Sch Med, NUS Tissue Engn Program, Singapore 119260, Singapore
4.Peking Univ, Ctr Biomat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
|Yin Jing,Yang Zheng,Cao Yong-ping,et al. Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity[J]. CHINESE MEDICAL JOURNAL,2011,124(24):4245-4253.|
|APA||Yin Jing,Yang Zheng,Cao Yong-ping,&Ge Zi-gang.(2011).Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity.CHINESE MEDICAL JOURNAL,124(24),4245-4253.|
|MLA||Yin Jing,et al."Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity".CHINESE MEDICAL JOURNAL 124.24(2011):4245-4253.|