IR@PKUHSC  > 北京大学第一临床医学院  > 骨科
学科主题临床医学
Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity
Yin Jing2; Yang Zheng3; Cao Yong-ping1; Ge Zi-gang2,4
关键词Osteoarthritis Chondrocyte Differentiation
刊名CHINESE MEDICAL JOURNAL
2011-12-20
DOI10.3760/cma.j.issn.0366-6999.2011.24.022
124期:24页:4245-4253
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]HUMAN ARTICULAR CHONDROCYTES ; GENE-EXPRESSION ; IN-VITRO ; II COLLAGEN ; PHENOTYPE ; CULTURE ; TISSUE ; VIVO ; QUANTIFICATION ; AGGRECANASE-1
英文摘要

Background There is a difficulty in evaluating the in vivo functionality of individual chondrocytes, and there is much heterogeneity among cartilage affected by osteoarthritis (OA). In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis.

Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan (GAG) content were measured. Cell morphology, proliferation, and gene expression of collagen type I, II, and X, aggrecan, matrix metalloproteinase 13 (MMP-13), and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture.

Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable.

Conclusions Expression of functional genes such as collagen type II and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration. Chin Med J 2011;124(24):4245-4253

语种英语
WOS记录号WOS:000299061400023
项目编号2011CB707500 ; 2012CB619102 ; 30772198 ; 30970881 ; 31011140348
资助机构National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57246
专题北京大学第一临床医学院_骨科
作者单位1.Peking Univ, Hosp 1, Dept Orthopaed, Beijing 100034, Peoples R China
2.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
3.Natl Univ Singapore, Yong Loo Lin Sch Med, NUS Tissue Engn Program, Singapore 119260, Singapore
4.Peking Univ, Ctr Biomat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yin Jing,Yang Zheng,Cao Yong-ping,et al. Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity[J]. CHINESE MEDICAL JOURNAL,2011,124(24):4245-4253.
APA Yin Jing,Yang Zheng,Cao Yong-ping,&Ge Zi-gang.(2011).Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity.CHINESE MEDICAL JOURNAL,124(24),4245-4253.
MLA Yin Jing,et al."Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity".CHINESE MEDICAL JOURNAL 124.24(2011):4245-4253.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yin Jing]的文章
[Yang Zheng]的文章
[Cao Yong-ping]的文章
百度学术
百度学术中相似的文章
[Yin Jing]的文章
[Yang Zheng]的文章
[Cao Yong-ping]的文章
必应学术
必应学术中相似的文章
[Yin Jing]的文章
[Yang Zheng]的文章
[Cao Yong-ping]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。