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学科主题: 药学
题名:
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma
作者: Yang, DY; Fushimi, H; Cai, SQ; Komatsu, K
关键词: Rheum ; matK gene ; amplification refractory mutation system (ARMS) analysis ; polymerase chain reaction-restriction ; fragment length polymorphism (PCR-RFLP) analysis ; Rhei Rhizoma ; identification
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2004-05-01
卷: 27, 期:5, 页:661-669
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: RIBOSOMAL-RNA GENE ; TRNK GENE ; MOLECULAR ANALYSIS ; SEQUENCE ; DNA ; CHINESE ; DRUGS
英文摘要:

Previously, we have determined marker nucleotides on the chloroplast matK gene to identify Rheum palma-tum, R. tanguticum and R. officinale used as Rhei Rhizoma officially. In the present study, we further developed a convenient and efficient identification method on the basis of marker nucleotides with Amplification Refractory Mutation System analysis. On the basis of the nucleotide substitutions at positions 367 and 937 among the three species on the matK gene, at each position two kinds of reverse primers with complementary 3′-terminal nucleotides were designed. Upon PCR amplification using three sets of primers and template DNA from each species, one or two fragments (202 bp or/and 770 bp) were detected. As the resultant three fragment profiles were species-specific, the procedure enabled us to classify the botanic origins of 22 drug samples of Rhei Rhizoma.

语种: 英语
WOS记录号: WOS:000221161200013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57258
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Toyama Med & Pharmaceut Univ, Inst Nat Med, Res Ctr Ethnomed, Toyama 9300194, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Yang, DY,Fushimi, H,Cai, SQ,et al. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2004,27(5):661-669.
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