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学科主题药学
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma
Yang, DY; Fushimi, H; Cai, SQ; Komatsu, K
关键词Rheum Matk Gene Amplification Refractory Mutation System (Arms) Analysis Polymerase Chain Reaction-restriction Fragment Length Polymorphism (Pcr-rflp) Analysis Rhei Rhizoma Identification
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2004-05-01
27期:5页:661-669
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]RIBOSOMAL-RNA GENE ; TRNK GENE ; MOLECULAR ANALYSIS ; SEQUENCE ; DNA ; CHINESE ; DRUGS
英文摘要

Previously, we have determined marker nucleotides on the chloroplast matK gene to identify Rheum palma-tum, R. tanguticum and R. officinale used as Rhei Rhizoma officially. In the present study, we further developed a convenient and efficient identification method on the basis of marker nucleotides with Amplification Refractory Mutation System analysis. On the basis of the nucleotide substitutions at positions 367 and 937 among the three species on the matK gene, at each position two kinds of reverse primers with complementary 3′-terminal nucleotides were designed. Upon PCR amplification using three sets of primers and template DNA from each species, one or two fragments (202 bp or/and 770 bp) were detected. As the resultant three fragment profiles were species-specific, the procedure enabled us to classify the botanic origins of 22 drug samples of Rhei Rhizoma.

语种英语
WOS记录号WOS:000221161200013
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57258
Collection北京大学药学院_天然药物学系
作者单位1.Toyama Med & Pharmaceut Univ, Inst Nat Med, Res Ctr Ethnomed, Toyama 9300194, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yang, DY,Fushimi, H,Cai, SQ,et al. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2004,27(5):661-669.
APA Yang, DY,Fushimi, H,Cai, SQ,&Komatsu, K.(2004).Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma.BIOLOGICAL & PHARMACEUTICAL BULLETIN,27(5),661-669.
MLA Yang, DY,et al."Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) and Amplification Refractory Mutation System (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei rhizoma".BIOLOGICAL & PHARMACEUTICAL BULLETIN 27.5(2004):661-669.
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