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学科主题: 临床医学
题名:
Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay
作者: Cui, Chanjuan1; Liu, Ping1; Feng, Zhenru1; Xin, Ruolei2; Yan, Cunling1; Li, Zhiyan1
关键词: Chemiluminescent microparticle immunoassay ; Human immunodeficiency virus ; Antigen ; Interfering factors
刊名: JOURNAL OF VIROLOGICAL METHODS
发表日期: 2015-03-15
DOI: 10.1016/j.jviromet.2014.07.026
卷: 214, 页:33-36
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]: HUMAN-IMMUNODEFICIENCY-VIRUS ; TESTING POPULATION ; ANTIBODY-ASSAYS ; UNITED-STATES ; REAL-TIME ; INFECTION ; ANTIGEN ; SPECIFICITY ; PERFORMANCE ; ALGORITHM
英文摘要:

Human immunodeficiency virus (HIV) screening assays have improved from single-antigen detection to detection of antigen-antibody combinations. However, concerns have been raised over the potential for false-positive results in antigen-antibody combination assays. The present study investigated the clinical effectiveness of HIV antigen/antibody (HIV Ag/Ab) combination screening by chemiluminescence microparticle immunoassay (CMIA) in over 88,000 samples from an HIV low-prevalence area of Beijing, China. The HIV Ag/Ab CMIA screening results were consistent with those obtained by Western blot and HIV-RNA testing, and had an accuracy of 99.74% (Kappa index = 0.98). False-positive results were more common for women affected by clinical interfering factors (e.g., kidney disease, tumors) than for men (80.95% vs. 15.09%, P < 0.001). When CMIA signal-to-cutoff ratio (S/CO) was 11.26, the sensitivity and specificity were highest (100%, 99.43%), and the area under the ROC curve (AUC) was 0.998. Specimens that were negative by CMIA (S/CO <1) were all negative by HIV-RNA testing. These results indicate that HIV Ag/Ab CMIA has a good clinical performance; however, some clinical interfering factors should be considered in HIV low-prevalence areas for their potential to skew testing results. (C) 2015 Published by Elsevier B.V.

语种: 英语
项目资助者: Abbott Laboratories, Wiesbaden, Germany ; Peking University First Hospital ; Abbott Laboratories
WOS记录号: WOS:000351654800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57261
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Lab Med, Beijing 100034, Peoples R China
2.Beijing Ctr Dis Prevent & Control, Beijing 100013, Peoples R China

Recommended Citation:
Cui, Chanjuan,Liu, Ping,Feng, Zhenru,et al. Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay[J]. JOURNAL OF VIROLOGICAL METHODS,2015,214:33-36.
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