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Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay
Cui, Chanjuan1; Liu, Ping1; Feng, Zhenru1; Xin, Ruolei2; Yan, Cunling1; Li, Zhiyan1
关键词Chemiluminescent Microparticle Immunoassay Human Immunodeficiency Virus Antigen Interfering Factors
刊名JOURNAL OF VIROLOGICAL METHODS
2015-03-15
DOI10.1016/j.jviromet.2014.07.026
214页:33-36
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]HUMAN-IMMUNODEFICIENCY-VIRUS ; TESTING POPULATION ; ANTIBODY-ASSAYS ; UNITED-STATES ; REAL-TIME ; INFECTION ; ANTIGEN ; SPECIFICITY ; PERFORMANCE ; ALGORITHM
英文摘要

Human immunodeficiency virus (HIV) screening assays have improved from single-antigen detection to detection of antigen-antibody combinations. However, concerns have been raised over the potential for false-positive results in antigen-antibody combination assays. The present study investigated the clinical effectiveness of HIV antigen/antibody (HIV Ag/Ab) combination screening by chemiluminescence microparticle immunoassay (CMIA) in over 88,000 samples from an HIV low-prevalence area of Beijing, China. The HIV Ag/Ab CMIA screening results were consistent with those obtained by Western blot and HIV-RNA testing, and had an accuracy of 99.74% (Kappa index = 0.98). False-positive results were more common for women affected by clinical interfering factors (e.g., kidney disease, tumors) than for men (80.95% vs. 15.09%, P < 0.001). When CMIA signal-to-cutoff ratio (S/CO) was 11.26, the sensitivity and specificity were highest (100%, 99.43%), and the area under the ROC curve (AUC) was 0.998. Specimens that were negative by CMIA (S/CO <1) were all negative by HIV-RNA testing. These results indicate that HIV Ag/Ab CMIA has a good clinical performance; however, some clinical interfering factors should be considered in HIV low-prevalence areas for their potential to skew testing results. (C) 2015 Published by Elsevier B.V.

语种英语
WOS记录号WOS:000351654800006
资助机构Abbott Laboratories, Wiesbaden, Germany ; Peking University First Hospital ; Abbott Laboratories
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57261
专题北京大学第一临床医学院
北京大学第一临床医学院_检验科
作者单位1.Peking Univ, Hosp 1, Dept Lab Med, Beijing 100034, Peoples R China
2.Beijing Ctr Dis Prevent & Control, Beijing 100013, Peoples R China
推荐引用方式
GB/T 7714
Cui, Chanjuan,Liu, Ping,Feng, Zhenru,et al. Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay[J]. JOURNAL OF VIROLOGICAL METHODS,2015,214:33-36.
APA Cui, Chanjuan,Liu, Ping,Feng, Zhenru,Xin, Ruolei,Yan, Cunling,&Li, Zhiyan.(2015).Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay.JOURNAL OF VIROLOGICAL METHODS,214,33-36.
MLA Cui, Chanjuan,et al."Evaluation of the clinical effectiveness of HIV antigen/antibody screening using a chemiluminescence microparticle immunoassay".JOURNAL OF VIROLOGICAL METHODS 214(2015):33-36.
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