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学科主题: 临床医学
题名:
Apelin-13 regulates proliferation, migration and survival of retinal Muller cells under hypoxia
作者: Lu, Qiang1,2; Jiang, Yan-rong1,2; Qian, Jing1,2; Tao, Yong1,2
关键词: Apelin-13 ; Muller cells ; VEGF ; Diabetes ; Diabetic retinopathy
刊名: DIABETES RESEARCH AND CLINICAL PRACTICE
发表日期: 2013-02-01
DOI: 10.1016/j.diabres.2012.09.045
卷: 99, 期:2, 页:158-167
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: DIABETIC-RETINOPATHY ; APJ RECEPTOR ; VASCULAR DEVELOPMENT ; GLUCOSE DEPRIVATION ; ENDOGENOUS LIGAND ; ANGIOGENIC FACTOR ; PEPTIDE ; VITREORETINOPATHY ; EXPRESSION ; MICE
英文摘要:

Aims: To investigate the effect of apelin-13 and the antagonist of apelin receptor (F13A) on retinal Muller cells in vitro.

Methods: Localization of apelin-13, GFAP and VEGF of Muller cells was detected by immunofluorescence. The effects of apelin-13 and F13A on cell function were assessed by MTT, spreading assay, apoptosis and Boyden chamber assay in vitro. Additionally, the mRNA and protein of apelin-13, GFAP and VEGF in cultured Muller cells were measured by real-time PCR and western blot.

Results: Under hypoxia, strong positive staining of apelin-13 was observed and particularly evident in the cytosol and around the nucleus. Exposure of Muller cells to hypoxia led to a progressive increase in mRNA (p < 0.01) and protein levels of apelin-13 (p < 0.01), with a maximal 2.5-fold and 2-fold stimulation at 4 h respectively, compared with normoxic controls. Treated with 0.1, 1, 10 and 100 ng/ml apelin-13, the protein level of GFAP (p < 0.01) and VEGF (p < 0.01) increased significantly in Muller cells in a dose-dependent manner after 24 h. Compared with the untreated cells, 10 ng/ml apelin-13 significantly promoted Muller cells migration (p < 0.01). Annexin/PI staining showed that apelin-13 can downregulate cell apoptosis with 30% to the most (p < 0.05). On the contrary, 20 ng/ml F13A-treated Muller cells spread less than the control cells, with significantly lower number of migrated cells and significantly higher rate of apoptosis.

Conclusions: The results of this study showed that apelin-13 modulated the proliferation, migration, spreading, survival of Muller cells and the expressions of GFAP and VEGF. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 20100001110073 ; 20110001110042 ; 7112142 ; 30901639 ; 81271027 ; 81260152 ; 2009B04
项目资助者: EFSD/CDS/Lilly grant ; research fund for the doctoral program of higher education of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Novel Program
WOS记录号: WOS:000317122400017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57264
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing, Peoples R China

Recommended Citation:
Lu, Qiang,Jiang, Yan-rong,Qian, Jing,et al. Apelin-13 regulates proliferation, migration and survival of retinal Muller cells under hypoxia[J]. DIABETES RESEARCH AND CLINICAL PRACTICE,2013,99(2):158-167.
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