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学科主题: 基础医学
题名:
Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL
作者: Gu, L; Yao, WJ; Yan, ZY; Xie, L; Sun, DG; Li, D; Zeng, Z; Wen, ZY
关键词: TFAR19 ; apoptosis ; MEL ; micropipette ; biorheological character
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2003-06-01
卷: 46, 期:3, 页:293-301
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要:

Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation.. Our biorheological study indicated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger osmotic fragility, lower cell surface charge density, increased elastic modulus K-1 which is inversely proportional to cells′ maximal deformation ability, obviously diminished surface viscosity mu with elastic modulus K-2 having no distinct changes. The above results provided some bases for recognizing the function of TFAR19 completely from the viewpoint of biorheology.

语种: 英语
WOS记录号: WOS:000183283000008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57268
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Sch Basic Med Sci, Hemarheol Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Mech & Engn Sci, Beijing 100871, Peoples R China

Recommended Citation:
Gu, L,Yao, WJ,Yan, ZY,et al. Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2003,46(3):293-301.
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