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学科主题基础医学
Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL
Gu, L; Yao, WJ; Yan, ZY; Xie, L; Sun, DG; Li, D; Zeng, Z; Wen, ZY
关键词Tfar19 Apoptosis Mel Micropipette Biorheological Character
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2003-06-01
46期:3页:293-301
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
英文摘要

Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation.. Our biorheological study indicated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger osmotic fragility, lower cell surface charge density, increased elastic modulus K-1 which is inversely proportional to cells′ maximal deformation ability, obviously diminished surface viscosity mu with elastic modulus K-2 having no distinct changes. The above results provided some bases for recognizing the function of TFAR19 completely from the viewpoint of biorheology.

语种英语
WOS记录号WOS:000183283000008
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57268
专题北京大学基础医学院
作者单位1.Peking Univ, Ctr Hlth Sci, Sch Basic Med Sci, Hemarheol Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Mech & Engn Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Gu, L,Yao, WJ,Yan, ZY,et al. Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2003,46(3):293-301.
APA Gu, L.,Yao, WJ.,Yan, ZY.,Xie, L.,Sun, DG.,...&Wen, ZY.(2003).Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,46(3),293-301.
MLA Gu, L,et al."Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 46.3(2003):293-301.
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