IR@PKUHSC  > 北京大学基础医学院  > 北京大学人类疾病基因研究中心
学科主题基础医学
CCDC134 interacts with hADA2a and functions as a regulator of hADA2a in acetyltransferase activity, DNA damage-induced apoptosis and cell cycle arrest
Huang, Jing1,2; Zhang, Li1,2; Liu, Wei1,2; Liao, Qinyuan1,2; Shi, Taiping1,2; Xiao, Lin1,2; Hu, Fanlei1,2; Qiu, Xiaoyan1,2
关键词CCDC134 hADA2a PCAF p53 Acetylation
刊名HISTOCHEMISTRY AND CELL BIOLOGY
2012-07-01
DOI10.1007/s00418-012-0932-5
138期:1页:41-55
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Microscopy
资助者National Natural Sciences Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Science and Technology Major Projects of New Drugs ; National Natural Sciences Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Science and Technology Major Projects of New Drugs
研究领域[WOS]Cell Biology ; Microscopy
关键词[WOS]TATA-BINDING PROTEIN ; HISTONE ACETYLATION ; P53 ACETYLATION ; HUMAN ADA3 ; COMPLEX ; ACTIVATION ; PCAF ; GCN5 ; TRANSCRIPTION ; COACTIVATORS
英文摘要

Human transcriptional adaptor hADA2a is an important component of the general control nonderepressible 5 (GCN5) histone acetyltransferase complex. Here, we report that coiled-coil domain containing 134 (CCDC134), a novel nuclear protein, binds to hADA2a and enhances the stability of the hADA2a protein in unstressed conditions. Furthermore, CCDC134 was found to participate in the p300/CBP-associated factor (PCAF) complex via hADA2a and affect the histone acetyltransferase activity of the complex. We also found that CCDC134 increased the PCAF-dependent K320 acetylation of p53 and p53 protein stability in the presence of hADA2a overexpression. Moreover, we demonstrated the biological significance of the interaction between CCDC134 and hADA2a. CCDC134 showed obvious nuclear accumulation after ultraviolet (UV) irradiation, and the knockdown of endogenous CCDC134 suppressed hADA2a-induced cell apoptosis activity and G1/S cell cycle arrest. Together, our findings indicate that CCDC134 might act as a novel regulator of hADA2a, and plays roles in the PCAF complex via hADA2a to affect its acetyltransferase activity and UV-induced DNA damage repair.

语种英语
所属项目编号30901303 ; 20090001120033 ; 2012ZX09103301-032
资助者National Natural Sciences Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Science and Technology Major Projects of New Drugs ; National Natural Sciences Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; National Science and Technology Major Projects of New Drugs
WOS记录号WOS:000305222300004
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57274
Collection北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Key Lab Immunol, Dept Immunol,Sch Basic Med Sci,Minist Hlth, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Huang, Jing,Zhang, Li,Liu, Wei,et al. CCDC134 interacts with hADA2a and functions as a regulator of hADA2a in acetyltransferase activity, DNA damage-induced apoptosis and cell cycle arrest[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2012,138(1):41-55.
APA Huang, Jing.,Zhang, Li.,Liu, Wei.,Liao, Qinyuan.,Shi, Taiping.,...&Qiu, Xiaoyan.(2012).CCDC134 interacts with hADA2a and functions as a regulator of hADA2a in acetyltransferase activity, DNA damage-induced apoptosis and cell cycle arrest.HISTOCHEMISTRY AND CELL BIOLOGY,138(1),41-55.
MLA Huang, Jing,et al."CCDC134 interacts with hADA2a and functions as a regulator of hADA2a in acetyltransferase activity, DNA damage-induced apoptosis and cell cycle arrest".HISTOCHEMISTRY AND CELL BIOLOGY 138.1(2012):41-55.
Files in This Item: Download All
File Name/Size DocType Version Access License
CCDC134 interacts wi(897KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Huang, Jing]'s Articles
[Zhang, Li]'s Articles
[Liu, Wei]'s Articles
百度学术
百度学术Similar articles in
[Huang, Jing]'s Articles
[Zhang, Li]'s Articles
[Liu, Wei]'s Articles
必应学术
必应学术Similar articles in
[Huang, Jing]'s Articles
[Zhang, Li]'s Articles
[Liu, Wei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: CCDC134 interacts with hADA2a and functions as a regulator of hADA2a in acetyltransferase activity, DNA damage-induced apoptosis and cell cycle arrest.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.