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学科主题基础医学
Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides
Guo, Jitao2; Nair, Manoj K. M.1; Galvan, Estela M.1; Liu, Shu-Lin2; Schifferli, Dieter M.1
关键词Yersinia pestis Minitransposon Polymyxin B Protamine LL-37
刊名MICROBIAL PATHOGENESIS
2011-09-01
DOI10.1016/j.micpath.2011.04.010
51期:3页:121-132
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Microbiology
研究领域[WOS]Immunology ; Microbiology
关键词[WOS]ESCHERICHIA-COLI K-12 ; PLASMINOGEN-ACTIVATOR PLA ; OUTER-MEMBRANE PROTEINS ; GRAM-NEGATIVE BACTERIA ; POLYMYXIN-RESISTANCE ; EPITHELIAL-CELLS ; DENDRITIC CELLS ; BUBONIC PLAGUE ; VI SECRETION ; VIRULENCE
英文摘要

Bacterial pathogens display a variety of protection mechanisms against the inhibitory and lethal effects of host cationic antimicrobial peptides (CAMPS). To identify Yersinia pestis genes involved in CAMP resistance, libraries of DSY101 (KIM6 caf1 pla psa) minitransposon Tn5AraOut mutants were selected at 37 degrees C for resistance to the model CAMPs polymyxin B or protamine. This approach targeted genes that needed to be repressed (null mutations) or induced (upstream P(BAD) insertions) for the detection of CAMP resistance, and predictably for improved pathogen fitness in mammalian hosts. Ten mutants demonstrated increased resistance to polymyxin B or protamine, with the mapped mutations pointing towards genes suspected to participate in modifying membrane components, genes encoding transport proteins or enzymes, or the regulator of a ferrous iron uptake system (feoC). Not all the mutants were resistant to both CAMPs used for selection. None of the polymyxin B- and only some protamine-resistant mutants, including the feoC mutant, showed increased resistance to rat bronchoalveolar lavage fluid (rBALF) known to contain cathelicidin and beta-defensin 1. Thus, findings on bacterial resistance to polymyxin B or protamine don′t always apply to CAMPs of the mammalian innate immune system, such as the ones in rBALF. (C) 2011 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000292443800005
项目编号RAI076695
资助机构NIH ; University of Pennsylvania Research Foundation ; University of Pennsylvania Veterinary Center for Infectious Disease ; China Scholarship Council (CSC)
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57281
专题北京大学基础医学院_病原生物学系
作者单位1.Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA
2.Peking Univ Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
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GB/T 7714
Guo, Jitao,Nair, Manoj K. M.,Galvan, Estela M.,et al. Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides[J]. MICROBIAL PATHOGENESIS,2011,51(3):121-132.
APA Guo, Jitao,Nair, Manoj K. M.,Galvan, Estela M.,Liu, Shu-Lin,&Schifferli, Dieter M..(2011).Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides.MICROBIAL PATHOGENESIS,51(3),121-132.
MLA Guo, Jitao,et al."Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides".MICROBIAL PATHOGENESIS 51.3(2011):121-132.
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