IR@PKUHSC  > 北京大学口腔医学院  > 牙周科
学科主题口腔医学
Hydrogen sulfide synergistically upregulates Porphyromonas gingivalis lipopolysaccharide-induced expression of IL-6 and IL-8 via NF-kappa B signalling in periodontal fibroblasts
Chi, Xiao-Pei1; Ouyang, Xiang-Ying1; Wang, Yi-Xiang2
关键词Hydrogen Sulfide Porphyrornonas Gingivalis Lps Inflammation Periodontitis
刊名ARCHIVES OF ORAL BIOLOGY
2014-09-01
DOI10.1016/j.archoralbio.2014.05.022
59期:9页:954-961
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Programme Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Programme Foundation
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]GENOMIC DNA-DAMAGE ; LIGAMENT FIBROBLASTS ; METHYL MERCAPTAN ; DISEASE ; CELLS ; APOPTOSIS ; PATHWAYS ; INFLAMMATION ; ACTIVATION ; CYTOKINES
英文摘要

Objectives: The periodontal pathogen Porphyromonas gingivalis produces hydrogen sulfide (H2S). H2S in the oral cavity is positively correlated with periodontitis but the mechanism by which H2S contributes to periodontal diseases is obscure. We investigated the effect of H2S in combination with P. gingivalis lipopolysaccharide (LPS) on expression of the pro-inflammatory cytoldnes interleukin (IL)-6 and IL-8 in periodontal fibroblasts and the underlying mechanism of action.

Material and methods: Gingival fibroblasts (GFs) and periodontal ligament cells (PDLCs) were treated with different concentrations of the H2S donor NaHS in the presence/absence of P. gingivalis LPS for different time periods. Expression of IL-6 and IL-8 was detected by real-time PCR and ELISA. The activity of nuclear factor-kappa B (NF-kappa B) signalling was investigated using western blotting, EMSA and pathway blockade assays.

Results: Real-time PCR and ELISA results showed that H2S not only upregulated expression of IL-6 and IL-8 at mRNA and protein levels in a dose- and time-dependent manner, but also aggravated P. gingivalis LPS-induced expression of IL-6 and IL-8 in GFs and PDLCs. Western blotting and EMSA showed that NF-kappa B signalling was activated by NaHS, P. gingivalis LPS, and both, which was in accordance with the expression levels of IL-6 and IL-8 in GFs and PDLCs. These results were confirmed using a NF-kappa B pathway blockade assay.

Conclusions: H2S synergistically upregulated P. gingivalis LPS-induced expression of IL-6 and IL-8 in GFs and PDLCs via activation of NF-kappa B signalling, which could promote the development of periodontitis. (C) 2014 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号81271150 ; 1019
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Programme Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Programme Foundation
WOS记录号WOS:000341481600011
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57292
专题北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Chi, Xiao-Pei,Ouyang, Xiang-Ying,Wang, Yi-Xiang. Hydrogen sulfide synergistically upregulates Porphyromonas gingivalis lipopolysaccharide-induced expression of IL-6 and IL-8 via NF-kappa B signalling in periodontal fibroblasts[J]. ARCHIVES OF ORAL BIOLOGY,2014,59(9):954-961.
APA Chi, Xiao-Pei,Ouyang, Xiang-Ying,&Wang, Yi-Xiang.(2014).Hydrogen sulfide synergistically upregulates Porphyromonas gingivalis lipopolysaccharide-induced expression of IL-6 and IL-8 via NF-kappa B signalling in periodontal fibroblasts.ARCHIVES OF ORAL BIOLOGY,59(9),954-961.
MLA Chi, Xiao-Pei,et al."Hydrogen sulfide synergistically upregulates Porphyromonas gingivalis lipopolysaccharide-induced expression of IL-6 and IL-8 via NF-kappa B signalling in periodontal fibroblasts".ARCHIVES OF ORAL BIOLOGY 59.9(2014):954-961.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chi, Xiao-Pei]的文章
[Ouyang, Xiang-Ying]的文章
[Wang, Yi-Xiang]的文章
百度学术
百度学术中相似的文章
[Chi, Xiao-Pei]的文章
[Ouyang, Xiang-Ying]的文章
[Wang, Yi-Xiang]的文章
必应学术
必应学术中相似的文章
[Chi, Xiao-Pei]的文章
[Ouyang, Xiang-Ying]的文章
[Wang, Yi-Xiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。