IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Subretinal Implantation of Retinal Pigment Epithelial Cells Derived From Human Embryonic Stem Cells: Improved Survival When Implanted as a Monolayer
Diniz, Bruno1,2; Thomas, Padmaja1; Thomas, Biju1,3; Ribeiro, Ramiro1,2,4; Hu, Yuntao1,5; Brant, Rodrigo1,2; Ahuja, Ashish1; Zhu, Danhong1; Liu, Laura1,6; Koss, Michael1,7; Maia, Mauricio2; Chader, Gerald1; Hinton, David R.1,3,8; Humayun, Mark S.1,3
关键词Age-related Macular Degeneration Retinal Pigment Epithelium Human Embryonic Stem Cells
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2013-07-01
DOI10.1167/iovs.12-11239
54期:7页:5087-5096
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]HUMAN BRUCHS MEMBRANE ; MACULAR DEGENERATION ; RCS RATS ; IMMUNE-RESPONSES ; VISUAL FUNCTION ; INTACT SHEETS ; ATHYMIC RAT ; NUDE RATS ; TRANSPLANTATION ; MODEL
英文摘要

PURPOSE. To evaluate cell survival and tumorigenicity of human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE) transplantation in immunocompromised nude rats. Cells were transplanted as a cell suspension (CS) or as a polarized monolayer plated on a parylene membrane (PM).

METHODS. Sixty-nine rats (38 male, 31 female) were surgically implanted with CS (n = 33) or PM (n = 36). Cohort subsets were killed at 1, 6, and 12 months after surgery. Both ocular tissues and systemic organs (brain, liver, kidneys, spleen, heart, and lungs) were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned. Every fifth section was stained with hematoxylin and eosin and analyzed histologically. Adjacent sections were processed for immunohistochemical analysis (as needed) using the following antibodies: anti-RPE65 (RPE-specific marker), anti-TRA-1-85 (human cell marker), anti-Ki67 (proliferation marker), anti-CD68 (macrophage), and anti-cytokeratin (epithelial marker).

RESULTS. The implanted cells were immunopositive for the RPE65 and TRA-1-85. Cell survival (P = 0.006) and the presence of a monolayer (P < 0.001) of hESC-RPE were significantly higher in eyes that received the PM. Gross morphological and histological analysis of the eye and the systemic organs after the surgery revealed no evidence of tumor or ectopic tissue formation in either group.

CONCLUSIONS. hESC-RPE can survive for at least 12 months in an immunocompromised animal model. Polarized monolayers of hESC-RPE show improved survival compared to cell suspensions. The lack of teratoma or any ectopic tissue formation in the implanted rats bodes well for similar results with respect to safety in human subjects.

语种英语
WOS记录号WOS:000322637000085
项目编号DR1-01444 ; EY03040 ; BEX 2326-11-6
资助机构California Institute of Regenerative Medicine ; Research to Prevent Blindness ; National Eye Institute ; CAPES Foundation
引用统计
被引频次:80[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57304
专题北京大学第三临床医学院_眼科
作者单位1.Doheny Eye Inst, Los Angeles, CA 90033 USA
2.Hosp Univ Curitiba, Dept Ophthalmol, Curitiba, Parana, Brazil
3.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
4.Univ Fed Sao Paulo, Dept Ophthalmol, Sao Paulo, Brazil
5.Univ So Calif, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA
6.Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
7.Goethe Univ Frankfurt, Dept Ophthalmol, D-60054 Frankfurt, Germany
8.Univ So Calif, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA
推荐引用方式
GB/T 7714
Diniz, Bruno,Thomas, Padmaja,Thomas, Biju,et al. Subretinal Implantation of Retinal Pigment Epithelial Cells Derived From Human Embryonic Stem Cells: Improved Survival When Implanted as a Monolayer[J]. INVESTIGATIVE OPHTHALMOLOGY &amp; VISUAL SCIENCE,2013,54(7):5087-5096.
APA Diniz, Bruno.,Thomas, Padmaja.,Thomas, Biju.,Ribeiro, Ramiro.,Hu, Yuntao.,...&Humayun, Mark S..(2013).Subretinal Implantation of Retinal Pigment Epithelial Cells Derived From Human Embryonic Stem Cells: Improved Survival When Implanted as a Monolayer.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,54(7),5087-5096.
MLA Diniz, Bruno,et al."Subretinal Implantation of Retinal Pigment Epithelial Cells Derived From Human Embryonic Stem Cells: Improved Survival When Implanted as a Monolayer".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 54.7(2013):5087-5096.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Diniz, Bruno]的文章
[Thomas, Padmaja]的文章
[Thomas, Biju]的文章
百度学术
百度学术中相似的文章
[Diniz, Bruno]的文章
[Thomas, Padmaja]的文章
[Thomas, Biju]的文章
必应学术
必应学术中相似的文章
[Diniz, Bruno]的文章
[Thomas, Padmaja]的文章
[Thomas, Biju]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。