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学科主题临床医学
Transcription factor Ap-1 mediates proangiogenic MIF expression in human endothelial cells exposed to Angiotensin II
Shan, Zhi-Xin1,3; Lin, Qiu-Xiong1; Yang, Min1; Zhang, Bin1; Zhu, Jie-Ning1; Mai, Li-Ping1; Deng, Chun-Yu1; Liu, Ju-Li1; Zhang, You-Yi2; Lin, Shu-Guang1; Yu, Xi-Yong1
关键词Angiotensin Ii Macrophage Migration Inhibitory Factor Ap-1 Endothelial Cells Angiogenesis
刊名CYTOKINE
2011
DOI10.1016/j.cyto.2010.09.009
53期:1页:35-41
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
关键词[WOS]MIGRATION-INHIBITORY FACTOR ; ACTIVATED PROTEIN-KINASE ; E-DEFICIENT MICE ; ATHEROSCLEROTIC PLAQUES ; SIGNAL-TRANSDUCTION ; PATHWAYS ; ANGIOGENESIS ; INSTABILITY ; BLOCKADE ; RECEPTOR
英文摘要

Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine associated with the atherosclerotic process and atherosclerotic plaque stability. MIF was shown to be highly expressed in advanced atherosclerotic lesions. Neutralizing MIF with a blocking antibody induced a regression of established atherosclerotic lesions.

In this study, we investigated the mechanism underlying the proangiogenic effect of MIF in human umbilical vein endothelial cells (HUVECs). We showed that MIF induced the expression of angiogenesis-related genes in HUVECs. We also showed that MIF induced tube formation of HUVECs in vitro and in vivo. Angiotensin II (Ang II) could specifically up-regulate MIF expression in HUVECs. Using a luciferase reporter assay, we demonstrated that the AP-1 response element in the 5′-UTR of the MIF gene played a role in Ang II-induced MIF expression. Small hairpin RNA (shRNA) targeting c-Jun, a component of AP-1, and the AP-1 inhibitor CHX both efficiently inhibited MIF expression. The consistent result of electrophoretic mobility shift assay (EMSA) showed that Ang II specifically increased AP-1 activation in HUVECs. Our results suggest that AP-1 mediates Ang II-induced MIF expression which contributes to atherosclerotic plaque destabilization in human endothelial cells. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000286862700008
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57319
专题北京大学第三临床医学院_心血管内科
作者单位1.Guangdong Acad Med Sci, Guangdong Gen Hosp, Res Ctr, Guangdong Prov Cardiovasc Inst, Guangzhou 510080, Guangdong, Peoples R China
2.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
3.Shantou Univ Med Coll, Dept Pharmacol, Shantou 515041, Peoples R China
推荐引用方式
GB/T 7714
Shan, Zhi-Xin,Lin, Qiu-Xiong,Yang, Min,et al. Transcription factor Ap-1 mediates proangiogenic MIF expression in human endothelial cells exposed to Angiotensin II[J]. CYTOKINE,2011,53(1):35-41.
APA Shan, Zhi-Xin.,Lin, Qiu-Xiong.,Yang, Min.,Zhang, Bin.,Zhu, Jie-Ning.,...&Yu, Xi-Yong.(2011).Transcription factor Ap-1 mediates proangiogenic MIF expression in human endothelial cells exposed to Angiotensin II.CYTOKINE,53(1),35-41.
MLA Shan, Zhi-Xin,et al."Transcription factor Ap-1 mediates proangiogenic MIF expression in human endothelial cells exposed to Angiotensin II".CYTOKINE 53.1(2011):35-41.
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