北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 消化内科  > 期刊论文
学科主题: 临床医学
题名:
Establishment of labeling primer reverse transcription in situ polymerase chain reaction and detection of hepatitis C virus in liver tissues
作者: Liu, YL; Du, SC; Tao, QM
关键词: in situ polymerase chain reaction ; hepatitis C virus
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2000-02-01
卷: 113, 期:2, 页:103-106
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: MESSENGER-RNA ; CELLS ; PCR ; CDNA
英文摘要:

Objective To establish bio-11-photosoralen (BP) labeling primer reverse transcription in situ polymerase chain reaction (PCR), and to detect the location and distribution of hepatitis C virus in 30 cases liver tissues embedded with paraffin.

Methods BPs were labeled in tymidine (T) position under ultraviolet lamp. The method was compared with indirect RT-in situ PCR and in situ hybridization for detecting hepatitis C virus (HCV) RNA.

Results Serum HCV PCR and southern blot showed that BP labeling psimer PCR was possible, and had a good specificity. The HCV positive rate was 53% (16/30) by indirect in situ PCR, 50% (15/30) positive specimens were found by BP labeling primer in situ PCR. Statistical analysis revealed P greater than or equal to 0.05 and the two methods had no dominant differences. Meanwhile, only 23% (7/30) positive signals were seen by in situ hybridization, which was lower than two in situ PCR(P less than or equal to 0.05). HCV was mainly located in hepatic-plasmas, and positive signals were found in monocytes and cholangiolar epithelia.

Conclusions Both indirect in situ PCR and BP labeling in situ PCR have good sensitivity and specificity for detecting HCV RNA of liver tissues. HCV RNA is located in hepatocytes, monocytes and cholangiolar epithelia.

语种: 英语
WOS记录号: WOS:000087114800002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57328
Appears in Collections:北京大学第二临床医学院_消化内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Med Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
2.Beijing Med Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Liu, YL,Du, SC,Tao, QM. Establishment of labeling primer reverse transcription in situ polymerase chain reaction and detection of hepatitis C virus in liver tissues[J]. CHINESE MEDICAL JOURNAL,2000,113(2):103-106.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, YL]'s Articles
[Du, SC]'s Articles
[Tao, QM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, YL]‘s Articles
[Du, SC]‘s Articles
[Tao, QM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace