IR@PKUHSC  > 北京大学第三临床医学院  > 肿瘤放疗科
学科主题临床医学
Oridonin-induced apoptosis in leukemia K562 cells and its mechanism
Liu, JJ; Huang, RW; Lin, DJ; Wu, XY; Peng, J; Pan, XL; Song, YQ; Lin, Q; Hou, M; Wang, DN; Chen, F; Zhang, MH
关键词Oridonin Apoptosis Bcl-2 Bax Telomerase
刊名NEOPLASMA
2005
52期:3页:225-230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TELOMERASE INHIBITION ; CANCER-THERAPY ; BCL-2 FAMILY ; DITERPENOIDS ; SURVIVAL
英文摘要

Oridonin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. Recently oridonin has been reported to have anti-tumor effects in a large variety of malignant diseases. In this study, we investigated the apoptotic inducing effect of oridonin in leukemia K562 cells and its mechanism. Cell growth inhibition was measured using a microculture tetrazolium assay, apoptosis was measured by flow cytometry and electron microscopy as well as by DNA fragmentation analysis. Telomerase activity was measured by TRAP-enzyme-linked immunosorbent assay, and the expression of Bcl-2 and Bax proteins was detected by western blot analysis. The results showed that oridonin could inhibit the proliferation and induce apoptosis on leukemia K562 cells remarkably. Telomerase activity as well as Bcl-2 expression was down-regulated, while Bax expression was up-regulated concurrently, when apoptosis ocurred. We therefore conclude that oridonin demonstrated anti-proliferative and apoptosis-inducing effects on K562 cells in vitro, and that changes in bcl-2 and bax protein levels as well as telomerase activity may play an important role in its mechanism of action.

语种英语
WOS记录号WOS:000229259000007
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57330
专题北京大学第三临床医学院_肿瘤放疗科
作者单位1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Hematol & Oncol, Guangzhou 510630, Guangdong, Peoples R China
2.Shandong Univ, QiLU Hosp, Dept Hematol & Oncol, Jinan 250012, Shandong, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Oncol, Beijing 100083, Peoples R China
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GB/T 7714
Liu, JJ,Huang, RW,Lin, DJ,et al. Oridonin-induced apoptosis in leukemia K562 cells and its mechanism[J]. NEOPLASMA,2005,52(3):225-230.
APA Liu, JJ.,Huang, RW.,Lin, DJ.,Wu, XY.,Peng, J.,...&Zhang, MH.(2005).Oridonin-induced apoptosis in leukemia K562 cells and its mechanism.NEOPLASMA,52(3),225-230.
MLA Liu, JJ,et al."Oridonin-induced apoptosis in leukemia K562 cells and its mechanism".NEOPLASMA 52.3(2005):225-230.
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